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2 protocols using anti phakt s473

1

Molecular Signaling Pathway Analysis

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Anti-MSI2 (#ab76148) and anti-VEGF-A (#ab46154) were obtained from Abcam (Cambridge, UK). Anti-VEGFR2 (#2479), anti-phVEGFR2 (#3817), anti-β-actin (#3700), anti-phAKT (T308) (#13038), anti-phAKT (S473) (#4060), anti-AKT total (#2920), anti-phGSKαβ (#9331), anti-GSKαβ total (#5676), anti-PTEN (#14642), anti-phERK (#4370), anti-ERK total (4695), anti-ph4EBP(#2855), anti-phP70SK (#9206), anti-P70S6K total (#2708), anti-rabbit HRP-linked (#7074), and anti-mouse HRP-linked (#7076) were obtained from Cell Signaling (Danvers, MA, USA). Doxycycline (#HY-N0565), hygromycin (HY-B0490), and puromycin (HY-B1743A) were obtained from MedChemExpress (Monmouth Junction, NJ, USA).
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2

Investigating VEGF/AKT/ERK Signaling Pathways

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Anti-MSI2 (#ab76148), anti-VEGF-A (#ab46154) were obtained from Abcam (Cambridge, UK), anti-VEGFR2 (#2479), anti-phVEGFR2 (#3817) anti-β-actin (#3700), anti-phAKT (T308) (#13038), anti-phAKT (S473) (#4060), anti-AKT total (#2920), anti-phGSKαβ (#9331), anti-GSKαβ total (#5676), anti-PTEN (#14642), anti-phERK (#4370), anti-ERK total (4695), anti-ph4EBP(#2855), anti-phP70SK (#9206), anti-P70S6K total (#2708) anti-rabbit HRP-linked (#7074), anti-mouse HRP-linked (#7076) were obtained from Cell Signaling (Danvers, MA). Doxycycline (#HY-N0565), hygromycin (HY-B0490) and puromycin (HY-B1743A) were obtained from MedChemExpress (Monmouth Junction, NJ).
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