Cy2 conjugated donkey anti rabbit igg h l

Manufactured by Jackson ImmunoResearch

Cy2-conjugated donkey anti-rabbit IgG (H + L) is a secondary antibody that is conjugated with the fluorescent dye Cy2. This antibody is designed to detect and bind to rabbit immunoglobulin G (IgG) antibodies, including both the heavy and light chains (H + L).

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Lab products found in correlation

Pi 1000, by Vector Laboratories (1 mentions) Ab152, by Merck Group (1 mentions) Ba 4001, by Vector Laboratories (1 mentions) Ba 9200, by Vector Laboratories (1 mentions) Ab84324, by Abcam (1 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) Cellsens software, by Olympus (1 mentions)

Spelling variants of this product

Anti-rabbit-Cy2 (11 citations) Donkey anti-Rabbit IgG (H+L); (4 citations) Rabbit anti-IgG (2 citations) Rabbit IgGs (2 citations)

2 protocols using cy2 conjugated donkey anti rabbit igg h l

Immunohistochemical analysis of Parkinson's disease

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Procedures for the preparation of brain tissues and immunohistochemistry are described in the Supplementary Methods (Additional file 1).
Primary antibodies (immunofluorescence): anti-TH (Millipore #AB152) 1:1000; anti-α-syn (Millipore #AB5334P) 1:1000; anti-α-syn clone 5G4 (AJ Roboscreen) 1:1000; anti-phospho S129 α-syn (Abcam ab59264) 1:1000. Secondary antibodies: Cy2-conjugated donkey anti-rabbit IgG (H + L) (Jackson ImmunoResearch Inc. #711–225-152) 1:1000; Cy3-conjugated F(ab’)₂ fragment donkey anti-sheep IgG (H + L) (Jackson ImmunoResearch Inc. #713–166-147) 1:1000.
Primary antibodies (DAB): anti-TH (Millipore #AB152 1:1000); anti-DAT (Millipore #MAB369) 1:4000; anti-HA-tag (Covance clone 16B12 #MMS-101P) 1:1000. Secondary antibodies: peroxidase Goat anti-rabbit IgG (Vector Laboratories #PI-1000) 1:200; peroxidase goat anti-mouse IgG (Vector Laboratories #BA-9200) 1:200; biotinylated rabbit anti-rat IgG (Vector Laboratories #BA-4001).

Bobela W., Nazeeruddin S., Knott G., Aebischer P, & Schneider B.L. (2017). Modulating the catalytic activity of AMPK has neuroprotective effects against α-synuclein toxicity. Molecular Neurodegeneration, 12, 80.

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Immunocytochemical Staining of Fibroblasts

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Skin fibroblast cells were fixed in 4% paraformaldehyde in PBS, permeabilized and blocked using 0.25% Triton X-100 and 5% serum in PBS for 30 min, and then incubated with ab84324 (Abcam) at 1 mg/ml for 3 h at room temperature. The secondary antibody used was Cy2-conjugated donkey anti-rabbit IgG (H+L) at a 1:500 dilution (Jackson ImmunoResearch). Actin cytoskeleton was stained with rhodamine phalloidin according to the manufacturer's instructions (Molecular Probes). DAPI was used for nuclear staining. Pictures were taken using an Olympus BX62 fluorescent microscope equipped with a DP72 camera and controlled by cellSens software (Olympus), which was also used for analysis and measurements.

Gur M., Cohen E.B., Genin O., Fainsod A., Perles Z, & Cinnamon Y. (2017). Roles of the cilium-associated gene CCDC11 in left-right patterning and in laterality disorders in humans. The International journal of developmental biology, 61(3-4-5).

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