Immunohistochemistry was performed either on alternate series of sections or in combination with ISH. Sections were washed in PBS, then in PGT (PBS with 0.2% gelatin and 0.25% Triton X-100) four times for 15 min. Sections were incubated overnight at 4°C with the following primary antibodies: anti-Tph2 (mouse monoclonal, 1/1000, Sigma-Aldrich), anti-5-HT (rabbit polyclonal, 1/1000, Sigma-Aldrich), anti–serotonin transporter (anti-SERT; rabbit polyclonal, 1/1000, Calbiochem). For fluorescence microscopy, sections were then incubated for 2 h at room temperature with the following secondary antibodies: donkey anti-rabbit 488 (1/200, The Jackson Laboratory), donkey anti-rabbit Cy3 (1/200, The Jackson Laboratory), donkey anti-mouse 488 (1/200, The Jackson Laboratory), donkey anti-mouse Cy3 (1/200, The Jackson Laboratory), or phalloidin 594 (1/40, Invitrogen). Sections were rinsed in PB, mounted in mowiol-Dabco (25 mg/ml), and stored at 4°C.
Anti tph2
Manufactured by Merck Group
Anti-TPH2 is a laboratory equipment product developed by Merck Group. It is designed to assist in the detection and measurement of tryptophan hydroxylase 2 (TPH2) enzyme levels. TPH2 is a key enzyme involved in the biosynthesis of serotonin, a neurotransmitter important for various physiological and behavioral functions. The Anti-TPH2 product provides a tool for researchers and scientists to study TPH2 expression and its role in neurological and psychiatric disorders.
Automatically generated - may contain errors
Lab products found in correlation
Microtome, by Leica camera (2 mentions)
Goat anti mouse, by Thermo Fisher Scientific (2 mentions)
Alexa tagged secondary antibody, by Thermo Fisher Scientific (2 mentions)
Donkey anti sheep, by Thermo Fisher Scientific (2 mentions)
Donkey anti rabbit, by Thermo Fisher Scientific (2 mentions)
Lsm 700 confocal microscope, by Zeiss (2 mentions)
Lsm 700, by Zeiss (2 mentions)
Phalloidin 594, by Thermo Fisher Scientific (1 mentions)
Donkey anti mouse 488, by Jackson ImmunoResearch (1 mentions)
Anti 5 ht, by Merck Group (1 mentions)
Donkey anti rabbit 488, by Jackson ImmunoResearch (1 mentions)
Donkey anti rabbit cy3, by Jackson ImmunoResearch (1 mentions)
Donkey anti mouse cy3, by Jackson ImmunoResearch (1 mentions)
Anti h3k27me3, by Cell Signaling Technology (1 mentions)
Anti eed, by Cell Signaling Technology (1 mentions)
3 protocols using anti tph2
1
Immunohistochemical Localization of Serotonergic Markers
2
Immunohistochemical Analysis of Brain Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were deeply anesthetized with Avertin intraperitoneal sodium pentobarbital (Apoteksbolaget AB) and perfused with room temperature phosphate buffer (PB) saline (PBS) through the ascending aorta, followed by ice-cold 4% paraformaldehyde. The brains were subsequently removed, postfixed in the same fixative for 16 to 18 hours, and cryoprotected for 24 to 48 hours in 30% sucrose at 4°C, before being cut on a Leica microtome at a thickness of 30 μm. Sections were permeabilized in 5% bovine serum albumin (BSA) in PBS-Tx100 (PBS with 0.5% Triton X-100), followed by primary antibody incubation at 4°C for 16 to 18 hours using sheep anti-TH (1:1000; catalog no. P60101-150, Pel-Freeze), anti-TPH2 (1:500; catalog no. T0678, Sigma-Aldrich), anti-H3K27me3 (1:500; catalog no. 9733, Cell Signaling Technology), and anti-EED (1:500; catalog no. 85322, Cell Signaling Technology). Fluorescent detection was done with an Alexa-tagged secondary antibody from Molecular Probes, donkey anti-sheep (1:500; catalog no. A21448), goat anti-mouse (1:500; catalog no. A21151), and donkey anti-rabbit (1:500; catalog no. A21206). Section images were obtained in an LSM-700 confocal microscope from Zeiss.
3
Neuronal Marker Immunohistochemistry in Rodent Brains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were deeply anesthetized with Avertin intraperitoneal sodium pentobarbital (Apoteksbolaget AB) and perfused with room-temperature phosphate buffer saline through the ascending aorta, followed by ice-cold 4% paraformaldehyde. The brains were subsequently removed, postfixed in the same fixative for 16-18 h and cryoprotected for 24-48 h in 30% sucrose at 4°C, before being cut on a Leica microtome at 30 µm thickness. Sections were permeabilized in 5% BSA in PBS-Tx100 (PBS with 0.5% Triton-X100), followed by primary antibody incubation at 4°C for 16-18 h using sheep anti-TH (1:1000, cat# P60101-150, Pel-Freeze), anti-TPH2 (1:500, cat# T0678, Sigma), anti-H3K27me3 (1:500, cat# 9733, CST), anti-EED (1:500, cat# 85322, CST). Fluorescent detection was done with an Alexatagged secondary antibody from Molecular Probes, donkey anti-sheep (1:500, cat# A21448), goat antimouse (1:500, cat# A21151), donkey anti-rabbit (1:500, cat# A21206). Section images were obtained in confocal microscope LSM-700 from Zeiss
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!