Log In Sign up
The largest database of trusted experimental protocols

Troglitazone

Manufactured by Bio-Techne
Visit Supplier
Sourced in United States

Troglitazone is a lab equipment product manufactured by Bio-Techne. It is a thiazolidinedione compound used for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Clozapine, by Bio-Techne (1 mentions) Methylisobutylxanthine, by Merck Group (1 mentions) Insulin, by Bio-Techne (1 mentions) Dorsomorphin, by Bio-Techne (1 mentions) Anti p62, by Cell Signaling Technology (1 mentions) Aicar, by Bio-Techne (1 mentions) Anti pparγ, by Santa Cruz Biotechnology (1 mentions) Gw9662, by Bio-Techne (1 mentions) Anti pampkα1 thr172, by Cell Signaling Technology (1 mentions) Anti acc, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Bodipy 493 503, by Merck Group (1 mentions) Sto 609, by Merck Group (1 mentions) Anti pacc ser79, by Cell Signaling Technology (1 mentions) Anti srebp, by Abcam (1 mentions) Anti pgc 1α, by Novus Biologicals (1 mentions) Anti mtor, by Cell Signaling Technology (1 mentions) Anti β tubulin, by Merck Group (1 mentions) Anti cd36, by Abcam (1 mentions) Anti ampk, by Cell Signaling Technology (1 mentions)

5 protocols using troglitazone

1

Liver Metabolism and Toxicology Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Troglitazone, clozapine, tienilic acid, and acetaminophen were purchased from Tocris Biosciences, Enzo Life Sciences, Sigma-Aldrich, and Sigma-Aldrich, respectively, and used without further purification. Other compounds and probes were synthesized and routes and analytical characterization are described in Supplementary Material. Mouse liver S9 fraction was obtained as an equal mix of male and female, samples from Xenotech, Lenexa, KS. All mouse studies were performed following protocols that received approval from The Scripps Research Institute–Institutional Animal Care and Use Committee office.
See Supplementary Methods sections for detailed experimental procedures.
Whitby L.R., Obach R.S., Simon G.M., Hayward M.M, & Cravatt B.F. (2017). Quantitative chemical proteomic profiling of the in vivo targets of reactive drug metabolites. ACS chemical biology, 12(8), 2040-2050.
+ Open protocol
+ Expand
2

Differentiation of MEFs to Adipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary MEFs were differentiated to adipocytes using the protocol of Fei et al.71 (link) with minor modifications. In brief, 1 × 106 MEFs (passage 3 or 4) were plated in six-well plates in DMEM-10% supplemented with 0.5 mM methylisobutylxanthine (Sigma, I7018), 1 M dexamethasone (DEX), 10 g/ml insulin and 10 M troglitazone (Tocris Bioscience, 3114/10). This media was kept for 3 days, and then replaced with DMEM-10% for another 2 days. After 5 days in culture 4-OHT was added to the culture twice for Mof deletion. For downstream analysis, cells were harvested before differentiation (MEFs) and after differentiation (iAdipo) followed or not by Mof deletion.
Pessoa Rodrigues C., Chatterjee A., Wiese M., Stehle T., Szymanski W., Shvedunova M, & Akhtar A. (2021). Histone H4 lysine 16 acetylation controls central carbon metabolism and diet-induced obesity in mice. Nature Communications, 12, 6212.
+ Open protocol
+ Expand
3

Regulation of AMPK and SREBP Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Capsaicin (CAP), AICAR, dorsomorphin (also named compound C), troglitazone and GW9662 were obtained from Tocris (Ellisville, MO, USA). BODIPY (493/503) and STO-609 (a CaMKKβ inhibitor) were from Sigma (St. Louis, MO, USA). Anti-pAMPKα1-Thr172, anti-AMPK, anti-pACC-Ser79, anti-ACC, anti-pSREBP-1c-Ser372, anti-pAKT-Ser473, anti-AKT, anti-pmTOR-Ser2248, anti-mTOR, anti-pS6-Thr389, anti-S6, anti-p62 from Cell Signaling Technology (Danvers, MA, USA), anti-SREBP, anti-CD36, anti-PPARα from Abcam (Cambridge, UK), anti-PPARγ from Santa Cruz Biotechnology (Dallas, TX, USA), anti-PGC-1α, anti-LC3B from Novus Biologicals (Abingdon, UK) and anti-β-tubulin from Sigma (St. Louis, MO, USA).
Bort A., Sánchez B.G., Mateos-Gómez P.A., Díaz-Laviada I, & Rodríguez-Henche N. (2019). Capsaicin Targets Lipogenesis in HepG2 Cells Through AMPK Activation, AKT Inhibition and PPARs Regulation. International Journal of Molecular Sciences, 20(7), 1660.
+ Open protocol
+ Expand
4

Adipocyte Differentiation Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oxysterols, oil red O stain and GW3965 were purchased from Sigma-Aldrich (St Louis, MO, USA). Dexamethasone (DEX), Insulin and 3-Isobutyl-1-methylxanthine (IBMX), cyclopamine and purmorphamine were purchased from Cayman chemical company (Ann Arbor, MI, USA). Troglitazone was purchased from Tocris Bioscience (Ellisville, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), Fetal bovine serum (FBS), Penicilin, streptomycin and L-glutamate were purchased from Mediatech, Inc (Manassas, VA, USA).
Moseti D., Regassa A., Chen C., O K, & Kim W.K. (2020). 25-Hydroxycholesterol Inhibits Adipogenic Differentiation of C3H10T1/2 Pluripotent Stromal Cells. International Journal of Molecular Sciences, 21(2), 412.
+ Open protocol
+ Expand
5

Characterization of Lymphoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
CB33, SUDHL4 and SUDHL6 cells provided by R. Dalla-Favera (Columbia University, NY) were maintained in IMDM (Life Technology), supplemented with 10% FBS (Gemini) and antibiotics. The HF1 follicular cell line provided by R. Levy (Stanford University, CA) was maintained in DMEM (Life Technology), supplemented with 10% FBS and antibiotics. Cells were tested negative for mycoplasma. Cells were not further authenticated. Antibodies: rabbit anti-MYC (XP) (Cell Signaling Technology); rabbit anti-FOXM1 and mouse anti-GAPDH (SantaCruz); rabbit anti-HMGA1, anti-ATF5, anti-NFYB, mouse anti-TFDP1 (Abcam). Alprostadil, Clemastine, Cytarabine and Troglitazone (Tocris), Econazole nitrate and Promazine hydrochloride (Sigma) were reconstituted in DMSO (Sigma).
Bisikirska B., Bansal M., Shen Y., Teruya-Feldstein J., Chaganti R, & Califano A. (2015). Elucidation and pharmacological targeting of novel molecular drivers of follicular lymphoma progression. Cancer research, 76(3), 664-674.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!