Ccl20 mip 3α

Manufactured by R&D Systems

Sourced in United States

CCL20/MIP-3α is a chemokine that is involved in the recruitment and migration of dendritic cells, lymphocytes, and other immune cells. It is produced by a variety of cells, including epithelial cells, endothelial cells, and some immune cells.

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Lab products found in correlation

Il 10, by Thermo Fisher Scientific (2 mentions) Ccl11 eotaxin 1, by R&D Systems (1 mentions) Gm csf, by R&D Systems (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Il 13, by R&D Systems (1 mentions) Il 12p70, by BioLegend (1 mentions) Il 23p19, by R&D Systems (1 mentions) Tnf α, by BioLegend (1 mentions) Prism software, by GraphPad (1 mentions) Il 1β, by BioLegend (1 mentions) Interleukin 6 (il 6), by Immunotools (1 mentions) P38 inhibitor sb203580, by Merck Group (1 mentions) Bay 11 7092, by Merck Group (1 mentions) Sp600125, by Merck Group (1 mentions) Pd98059, by Merck Group (1 mentions) Cxcl8 il 8, by R&D Systems (1 mentions)

3 protocols using ccl20 mip 3α

Analyzing Chemokine Signaling Pathways

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Human recombinant CCL2/MCP-1, CCL20/MIP-3α and CXCL8/IL-8 proteins, and polyclonal antihuman CCL2/MCP-1, CCL20/MIP-3α and CXCL8/IL-8 antibodies were obtained from R&D Systems (Minneapolis, MN, USA). Control nonspecific IgG from goat serum was purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). The NF-κB inhibitor BAY 11-7092, c-Jun N-terminal Kinase (JNK) inhibitor SP600125, p38 inhibitor SB203580 and were purchased from Sigma-Aldrich, Co. Extracellular signal-regulated Kinase (ERK) inhibitor PD98059 was obtained from Merck (Darmstadt, Germany). Exo-mCherry (mChrry-loaded exosome) and Exo-srIκB (srIκB-loaded exosome) were obtained from Cellex Life Sciences Inc. (Daejeon, Korea) [32] .

Jung Y., Ahn S.H., Park H., Park S.H., Choi K., Choi C., Kang J.L, & Choi Y.H. (2018). MCP-1 and MIP-3α Secreted from Necrotic Cell-Treated Glioblastoma Cells Promote Migration/Infiltration of Microglia. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(3).

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Cytokine Profiling of BAL and Lung

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Supernatants from BAL and lung homogenates were collected and then used undiluted in commercial ELISA kits for KC/mCXCL1, CCL20/MIP-3α, TSLP, GM-CSF, CCL11/eotaxin-1, IL-4, IL-5 and IL-13 (R&D Systems) according to the manufacturer’s protocol.

Causton B., Ramadas R.A., Cho J.L., Jones K., Pardo-Saganta A., Rajagopal J., Xavier R.J, & Medoff B.D. (2015). CARMA3 Is Critical for the Initiation of Allergic Airway Inflammation. The Journal of Immunology Author Choice, 195(2), 683-694.

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Cytokine Secretion Profiling of Intestinal and Immune Cells

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IL-8 (IL-8/CXCL8 ImmunoTools, Friesoythe, Germany) and chemokine (C–C motif) ligand 20 (CCL20/MIP-3α, R&D Systems, Minneapolis, MN, USA) secretion by intestinal epithelial HT29 cells after 7 h of infection was analyzed by ELISA assays according to the manufacturer’s instructions.
Supernatants of MDM were collected at 8 h post-infection and analyzed for TNF-α, (BioLegend, San Diego, CA, USA), IL-6 (ImmunoTools) and IL-10 (Thermo Fisher, Rockford, IL, USA) by ELISA assays. The levels of IL-1β (BioLegend), IL-12p70 (BioLegend), IL-23p19 (R&D Systems) and IL-10 (Thermo Fisher) in supernatants of infected MoDC were analyzed after 24 h by ELISA assays. The Elisa plates were read on microplate reader (SAFAS MP96), and data were analyzed with Prism software (version 7; GraphPad Software, Inc., La Jolla, CA, USA).

Leccese G., Bibi A., Mazza S., Facciotti F., Caprioli F., Landini P, & Paroni M. (2020). Probiotic Lactobacillus and Bifidobacterium Strains Counteract Adherent-Invasive Escherichia coli (AIEC) Virulence and Hamper IL-23/Th17 Axis in Ulcerative Colitis, but Not in Crohn’s Disease. Cells, 9(8), 1824.

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