For immunoblots, proteins were probed with one of the following primary antibodies in either 5% non-fat milk or 5% bovine serum albumin: anti-HEC1 (#3613, 1:1000 dilution) and anti-NEK3 (#37636, 1:800 dilution) antibodies from Abcam; anti-XPO1 (#46249, 1:1000 dilution), anti-phospho-Akt (Ser473) (#4051, 1:1000 dilution), and anti-GAPDH (#5174, 1:3000 dilution) from Cell Signaling Technology; anti-NUP205 (Santa Cruz Biotechnology, 1:200 dilution), and anti-tubulin (acetylated) (T6793, Sigma-Aldrich, 1:1000 dilution for WB).
Anti nek3
Manufactured by Abcam
Anti-NEK3 is a primary antibody targeting the NEK3 protein. NEK3 is a serine/threonine protein kinase that plays a role in regulating cell cycle and microtubule dynamics. This antibody can be used for applications such as Western blotting and immunoprecipitation to detect and study the NEK3 protein.
Automatically generated - may contain errors
2 protocols using anti nek3
1
Antibody Immunoblot Screening Protocol
2
Protein Expression Profiling in Breast Cancer
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Expression of proteins aromatase, NEK3, PIAS3, and prolactin was investigated in breast cancer cell lines BT-549, EFM192A, HCC1937, and MDA-MB-231 by intracellular staining with anti-aromatase (Bioss, Cat#bs-1292R), anti-NEK3 (Abcam, Cat#83221), anti-PIAS3 (Abcam, Cat#ab77231) and anti-prolactin (Lifespan Biosciences, Cat#LS-C209024) antibodies, with analysis by flow cytometry. Tumor cells were harvested and washed twice in R10, once in PBS + 2% FCS. Thereafter, cells were permeabilized and fixated with the Transcription Factor Buffer Set (BD, Cat#562574) and stained for 30 min at 4°C with specific antibodies or isotype/stain control antibodies. Followingly, cells were washed twice in PBS + 2% FCS and stained with secondary antibodies when relevant, washed twice in PBS + 2% FCS and finally fixated in 1% paraformaldehyde (PFA, Santa Cruz Biotechnology, Cat#sc-281692) until analysis.
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