FDG was prepared through nucleophilic 18F-fluorination and hydrolysis of mannose triflate by the Stanford Cyclotron Radiochemistry Facility. 18F was made in a GE PETtrace cyclotron and the production was performed via cassette-based automated synthetic module (FASTlab, GE Healthcare). Quality control tests were performed according to USP823. The radiotracer was used within 8 h after production due to its short halftime (τ1/2 = 1.8 h). Radioactivity was measured using a dose calibrator (Atomlab 400, Biodex) prior to each experiment.
Atomlab 400
Manufactured by Mirion Technologies
Sourced in United States
The Atomlab 400 is a radiation detection and measurement device designed for use in laboratory settings. It is capable of accurately measuring radiation levels and provides essential data for radiation safety and analysis purposes.
Automatically generated - may contain errors
6 protocols using atomlab 400
1
FDG Radiochemical Synthesis Protocol
2
Radionuclide Labeling of Elastin-like Polypeptide
3
Automated [18F]FDG Radiopharmaceutical Production
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[18F]FDG was prepared through
nucleophilic 18F-fluorination and hydrolysis of mannose
triflate by the Stanford Cyclotron Radiochemistry Facility. 18F was made in a GE PETtrace cyclotron and the production was performed
via cassette-based automated synthetic module (FASTlab, GE Healthcare).
Quality control tests were performed according to USP823. The radiotracer
was used within 8 h after production due to its short halftime (τ1/2 = 1.8 h). Radioactivity was measured with a dose calibrator
(Atomlab 400, Biodex) prior to each experiment.
nucleophilic 18F-fluorination and hydrolysis of mannose
triflate by the Stanford Cyclotron Radiochemistry Facility. 18F was made in a GE PETtrace cyclotron and the production was performed
via cassette-based automated synthetic module (FASTlab, GE Healthcare).
Quality control tests were performed according to USP823. The radiotracer
was used within 8 h after production due to its short halftime (τ1/2 = 1.8 h). Radioactivity was measured with a dose calibrator
(Atomlab 400, Biodex) prior to each experiment.
4
Single-Cell Radioactivity Quantification
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An automatic gamma counter system (Hidex) was utilized to measure the radioactivity of individual cells. Single radiolabeled cells were transferred to 250 μl microcentrifuge tubes and loaded into racks for automated gamma counting. Each tube contained 100 μl fresh media and 1 μl droplet from the single-cell dispenser (composed of 99.7% PBS sheath fluid, 0.3% cell culture media solution). As a result, the radioactivity in each tube mainly originated from the single cell as the efflux in the cell solution was highly diluted. The radioactivity values in counts per second were converted to Bq through a calibration curve constructed from the measurement of serially diluted FDG solutions and a standard dose calibrator (Biodex, Atomlab 400). To confirm the singularity of dispensed single cells, we divided the solution in each tube equally into two or three vials and measured the activity of each fraction by gamma counting and PET imaging. The rationale for this experiment is that the contents of the single cell cannot be fractionated into multiple vials.
5
Iodine-131 Labeling of Elastin-like Polypeptides
6
Siemens Inveon PET Platform Imaging
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The Siemens Inveon PET platform was used to perform imaging experiments. Its detector consists of lutetium oxyorthosilicate (LSO) crystals coupled through a light-guide to position sensitive photo-multiplier tubes. The LSO crystals are arranged in 16 detector blocks, each with 4 detectors axially which are divided into 20 × 20 crystal arrays. The ring diameter is 16.1 cm and the axial length 12.7 cm, with individual crystal sizes of 1.5 × 1.5 × 10 mm3. For the acquisitions, an energy window of 350–650 keV and a coincidence timing window of 3.432 μs were used.
Prior to injection into the phantoms, the radionuclide activity was measured in an Atomlab 400 dose calibrator (Biodex Medical Systems, NY, USA). Emission data was acquired in list mode, and the Inveon Acquisition Workplace (v. 1.5.0.28) was used to bin the data into sinograms and reconstruct the images. Images were reconstructed with three image reconstruction procedures available, using the default parameters. The reconstruction procedures were 2D FBP (Fourier rebinning, Nyquist cut-off 0.5), OSEM3D-MAP (2 OSEM3D iterations, 18 MAP iterations, 1.5-mm requested resolution), and OSEM2D (4 iterations).
Prior to injection into the phantoms, the radionuclide activity was measured in an Atomlab 400 dose calibrator (Biodex Medical Systems, NY, USA). Emission data was acquired in list mode, and the Inveon Acquisition Workplace (v. 1.5.0.28) was used to bin the data into sinograms and reconstruct the images. Images were reconstructed with three image reconstruction procedures available, using the default parameters. The reconstruction procedures were 2D FBP (Fourier rebinning, Nyquist cut-off 0.5), OSEM3D-MAP (2 OSEM3D iterations, 18 MAP iterations, 1.5-mm requested resolution), and OSEM2D (4 iterations).
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