Human sensory neuron differentiation was performed using a modified version of a published protocol [12 (link); 13 (link)]. In brief, iPSCs were seeded on a Matrigel coated plate and maintained in mTeSR medium (Stem Cell Technology). Neural induction started once the cells reached 80% confluence by adding KSR medium consisting of Knockout DMEM and Knockout Serum Replacement (Gibco). 100nM LDN-193189 and 10μM SB-431054 (Tocris) were added from day 0 to 5 (DD0-DD5). N2 media consisting of Neurobasal media (Gibco) and 1X N2 Supplement (Gibco) were added starting at day 4 in concentration increments of 25% every other day, reaching 100% at day 10. Nociceptor induction started on day 2 by adding 3μM CHIR99021, 10μM SU5402, and 10μM DAPT (Tocris). Starting on day 11 (DD10) the cells were maintained in N2 media supplemented with 25ng/ml of human β-NGF, BDNF, and GDNF (Peprotech).
Sb 431054
Manufactured by Bio-Techne
SB-431054 is a potent and selective inhibitor of the Transforming Growth Factor-beta (TGF-β) type I receptor ALK5. It blocks the TGF-β signaling pathway by inhibiting the phosphorylation and activation of Smad2 and Smad3, which are key intracellular mediators of the TGF-β signal.
Automatically generated - may contain errors
Lab products found in correlation
Su5402, by Bio-Techne (2 mentions)
Knockout dmem, by Thermo Fisher Scientific (2 mentions)
N2 supplement, by Thermo Fisher Scientific (2 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (2 mentions)
Neurobasal media, by Thermo Fisher Scientific (2 mentions)
Chir99021, by Bio-Techne (2 mentions)
Ldn193189, by Bio-Techne (2 mentions)
Mtesr medium, by STEMCELL (2 mentions)
2 protocols using sb 431054
1
Differentiation of Human Sensory Neurons
2
Differentiation of Human Sensory Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human sensory neuron differentiation was performed using a modified version of a published protocol.12 (link),13 (link) In brief, iPSCs were seeded on a Matrigel-coated plate and maintained in mTeSR medium (Stem Cell Technology). Neural induction started once the cells reached 80% confluence by adding KSR medium consisting of Knockout DMEM and Knockout Serum Replacement (Gibco, Waltham, MA). 100nM LDN-193189 and 10μM SB-431054 (Tocris, Minneapolis, MN) were added from day 0 to 5 (DD0-DD5). N2 media consisting of Neurobasal media (Gibco) and 1X N2 Supplement (Gibco) were added starting at day 4 in concentration increments of 25% every other day, reaching 100% at day 10. Nociceptor induction started on day 2 by adding 3µM CHIR99021, 10µM SU5402, and 10µM DAPT (Tocris). Starting on day 11 (DD10), the cells were maintained in N2 media supplemented with 25ng/mL of human β-NGF, BDNF, and GDNF (PeproTech).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!