Interferon γ apc xmg1.2

Manufactured by Thermo Fisher Scientific

Interferon-γ APC (XMG1.2) is a laboratory reagent used for the detection and enumeration of interferon-gamma (IFN-γ) producing cells in flow cytometry applications. It is a monoclonal antibody conjugated with the fluorescent dye Allophycocyanin (APC).

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Lab products found in correlation

Cd4 percp cy5.5 rm4 5, by Thermo Fisher Scientific (1 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions) Cytofix cytoperm plus fixation permeabilization kit, by BD (1 mentions) Violet proliferation dye 450, by BD (1 mentions) Aqua live dead dye, by Thermo Fisher Scientific (1 mentions) Cd8α apc cy7, by Thermo Fisher Scientific (1 mentions) Cd3 fitc 17a2, by Thermo Fisher Scientific (1 mentions) Cd8α efluor450, by Thermo Fisher Scientific (1 mentions) Cd11b percpcy5.5 m1 70, by Cytek Biosciences (1 mentions) Foxp3 fitc fjk 16s, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions)

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Interferon-γ (36 citations) γ (XMG1.2) (6 citations)

2 protocols using interferon γ apc xmg1.2

Multi-parametric Flow Cytometry for T-cell and Regulatory Immune Responses

Cited 2 times

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ICS for T-cell cytokines was performed as described,^{2 (link)} using the Cytofix/Cytoperm Plus fixation/permeabilization kit (BD Biosciences), and antibodies to CD4 PE-Cy7 (RM4-5), IL-17A PerCP-Cy5.5 (eBio17B7), and interferon-γ APC (XMG1.2) (all eBioscience). For staining of regulatory T cells (Treg), the FoxP3 staining buffer set (eBioscience) and antibodies to CD4 PerCP-Cy5.5 (RM4-5), CD25 APC (PC61.5), and FoxP3 PE (FJK-16 s) were used. Intracellular IL-10 expression was analyzed by flow cytometry as previously described,^{9 (link)} using the Cytofix/Cytoperm Plus fixation/permeabilization kit (BD Biosciences). The following antibodies were used: CD19 PerCP-Cy5.5 (eBio1D3), CD45 PE-Cy7 (30-F11), CD11b APC-Cy7 (M1/70), IL-10 APC (JES5-16E3) (all from eBioscience), and B220 (CD45R) FITC (RA3-6B2) (BD Biosciences). An isotype- and fluorochrome-matched control antibody (IgG2b κ APC; eBioscience) was used to assess nonspecific staining for IL-10.

Lehmann-Horn K., Sagan S.A., Winger R.C., Spencer C.M., Bernard C.C., Sobel R.A, & Zamvil S.S. (2016). CNS accumulation of regulatory B cells is VLA-4-dependent. Neurology® Neuroimmunology & Neuroinflammation, 3(2), e212.

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Multiparametric Flow Cytometry for Immune Cell Profiling

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Flow cytometry was utilized to determine cell phenotype and cell proliferation. Cells were stained for surface markers using fluorochrome conjugated antibodies for 30 minutes on ice, washed, acquired using FACSCantoII (BD Biosciences) and analyzed using FlowJo v10.4.2 (Tree Star LLC). Antibodies and their specific clones used were: CD3-FITC (17A2, eBioscience), CD3-PerCPCy5.5 (145–2C11, Tonbo Biosciences), CD4-Pacific Blue (RM4–5, eBioscience), CD4-PerCPCy5.5 (RM4–5, Tonbo Biosciences), CD8α-APC-Cy7 (53–6.7, eBioscience), CD8α-eFluor450 (53–6.7, eBioscience), CD11b-PercpCy5.5 (M1/70, Tonbo Biosciences), CD11c-APC-eFluor780 (N418, eBioscience), B220-PECy7 (RA3–6B2, eBioscience), CD44-PECy7(IM7, eBioscience), Foxp3-FITC (FJK-16s, eBioscience), I-A^b-APC(AF6–120.1, eBioscience), interferon-γ-APC (XMG1.2, eBioscience), violet proliferation dye (VPD450, BD Biosciences), and aqua live/dead dye (Molecular Probes).

Shah S., Daneshmandi S., Hughes K.R., Yu S., Bedoya A.M., Shea L.D, & Luo X. (2019). Optimizing PLG nanoparticle-peptide delivery platforms for transplantation tolerance using an allogeneic skin transplant model. Biomaterials, 210, 70-82.

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