Ulex europaeus lectin

Manufactured by Merck Group

Sourced in United Kingdom

Ulex europaeus lectin is a carbohydrate-binding protein derived from the seeds of the gorse plant (Ulex europaeus). It has a specific affinity for the sugar N-acetylgalactosamine and is commonly used as a histochemical reagent in biological research and diagnostic applications.

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Lab products found in correlation

Collagenase type 5, by Merck Group (1 mentions) Dynabead, by Thermo Fisher Scientific (1 mentions) Actinomycin d, by Merck Group (1 mentions) Dnase 1, by Qiagen (1 mentions)

Close spelling variants of this product

Ulex europaeus (5 citations) Ulex europaeus agglutinin I (lectin, (4 citations) Atto 488-conjugated Ulex europaeus lectin (3 citations) Ulex-lectin (2 citations) Ulex europaeus agglutinin (lectin, (2 citations) Ulex europaeus 1 lectin (2 citations)

3 protocols using ulex europaeus lectin

Quantifying Circulating Endothelial Cells

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Circulating endothelial cells (CECs) were extracted from whole blood by CD146-coated immunomagnetic beads (5050-P, BioCytex) using an international consensus protocol (45 (link)). The extracted CECs were counted using a Nageotte chamber under a fluorescence microscope and were defined as Ulex europaeus lectin (L9006, Sigma-Aldrich) bright cells that were greater than 10 μm in size, with 5 or more magnetic beads attached.

Zhou H., Hong Y., Scoto M., Thomson A., Pead E., MacGillivray T., Hernandez-Gerez E., Catapano F., Meng J., Zhang Q., Hunter G., Shorrock H.K., Ng T.K., Hamida A., Sanson M., Baranello G., Howell K., Gillingwater T.H., Brogan P., Thompson D.A., Parson S.H, & Muntoni F. (2022). Microvasculopathy in spinal muscular atrophy is driven by a reversible autonomous endothelial cell defect. The Journal of Clinical Investigation, 132(21), e153430.

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Isolation of Endothelial Cells from Diverse Tissues

Cited 1 time

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The process is summarized in Figure 1A and based on the protocol published in (14 (link)). Briefly, tissue was minced and digested in DMEM containing 2 mg ml⁻¹ collagenase type V (Sigma, Gillingham, UK), 7.4 mg ml⁻¹ actinomycin D (Sigma) and 30kU ml⁻¹ DNase I (Qiagen, Crawley, UK), shaking at 37°C for 2 hours (CRC and Colon) or 1.5 hours (RCC, Kidney, CRM and Liver). Endothelial cells were isolated from the digested single cell suspension by positive magnetic selection using 1.4×10⁻⁷ g⁻¹ streptavidin coated Dynabeads^® (Invitrogen, Paisley, UK) conjugated to biotinylated Ulex europaeus lectin (Sigma).

Wragg J.W., Finnity J.P., Anderson J.A., Ferguson H.J., Porfiri E., Bhatt R.I., Murray P.G., Heath V.L, & Bicknell R. (2016). MCAM and LAMA4 are highly enriched in tumor blood vessels of renal cell carcinoma and predict patient outcome. Cancer research, 76(8), 2314-2326.

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Immunomagnetic Isolation of Circulating Endothelial Cells

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CECs were extracted from whole blood using CD146-coated immunomagnetic beads using an international consensus protocol [25 (link), 26 (link)]. CECs extracted in this way were counted using a Nageotte chamber on a fluorescence microscope and were defined as Ulex europaeus lectin (Sigma-Aldrich, Doset, UK) bright cells that were > 10 μm in size, with five or more magnetic beads attached.

Hong Y., Eleftheriou D., Klein N.J, & Brogan P.A. (2015). Impaired function of endothelial progenitor cells in children with primary systemic vasculitis. Arthritis Research & Therapy, 17, 292.

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