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Anti cldn4

Manufactured by Thermo Fisher Scientific
Sourced in United States

Anti-CLDN4 is a laboratory reagent used for the detection and quantification of Claudin-4 (CLDN4) protein in biological samples. Claudin-4 is a tight junction protein that plays a role in the formation and regulation of cell-cell adhesion. The Anti-CLDN4 reagent can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and ELISA, to measure the expression levels of CLDN4 in research and diagnostic applications.

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2 protocols using anti cldn4

1

Immunostaining Markers for Cell Types

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Anti-CLDN4 (mouse), anti-CLDN5 (mouse (tissues) and rabbit (cell culture)), anti-human ZO1 (rabbit), and anti-GFAP (rat) were from Invitrogen (Carlsbad, California, USA). Anti-CDH5 (goat) was from R&D systems (Minneapolis, Minnesota, USA). Anti-human CDH5 (mouse) and anti-human DHH (H-85) (rabbit) were from Santa Cruz Biotech (Santa Cruz, California, USA). Anti-FGB (rabbit) and anti-human PECAM1 (mouse) were from Dako (Carpinteria, California, USA). Anti-ALB (sheep) and anti-MBP (rat) were from Abcam (Cambridge, Massachusetts, USA). Anti-CD45 (rat) was from eBioscience (San Diego, California, USA). Anti-RNA binding fox-1 homolog 3 also known as neuronal nuclei antigen (NEUN) (rabbit) and anti-AQP4 (rabbit) were from Millipore (Billerica, Massachusetts, USA). Anti-LAM (rabbit) was from Sigma Aldrich (St. Louis, Missouri, USA). Anti-ZO1 (rabbit) was from Life Technologies (Grand Island, New York, USA). Anti-FOXO1 (rabbit), anti-p-FOXO1 (rabbit), and anti-β-ACTIN (rabbit) were from cell signaling (Danvers, Massachusetts, USA).
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2

Multicancer Biomarker Targeting via QDs

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Antibodies were
conjugated to the lipid coated QDs using an FcγRI group attached
to a his-tag. Recombinant mouse FcγRI/CD64 (R&D Systems,
cat# 2074-FC-050) with a C-terminal 6xHis-tag constituted at 500 μg
mL–1 in sterile PBS was added to NTA(Ni)-terminated
QDs for 30 min at RT. The concentration of the 6xHis-tag Fc-receptor
was calculated to give three Fc-receptors per QD. The resulting solution
was passed through a syringe filter with a 200 nm polytetrafluoroethylene
membrane to remove any aggregates.
Targeting experiments were
performed by conjugating antibodies of four pancreatic cancer biomarkers:57 (link) Claudin-4 (anti-CLDN4, #329400, Invitrogen),
mesothelin (anti-MSLN, #354200, Invitrogen), mucin-4 (anti-MUC4, #354900,
Invitrogen), and cadherin-11 (anti-CDH11, ab151446, Abcam). Antibodies
as supplied (100 μg in 200 μL) were added to a suspension
of the QD-FcR complex to give three antibodies per QD and incubated
for 1 h at RT.
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