Anti cldn4
Anti-CLDN4 is a laboratory reagent used for the detection and quantification of Claudin-4 (CLDN4) protein in biological samples. Claudin-4 is a tight junction protein that plays a role in the formation and regulation of cell-cell adhesion. The Anti-CLDN4 reagent can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and ELISA, to measure the expression levels of CLDN4 in research and diagnostic applications.
Lab products found in correlation
2 protocols using anti cldn4
Immunostaining Markers for Cell Types
Multicancer Biomarker Targeting via QDs
conjugated to the lipid coated QDs using an FcγRI group attached
to a his-tag. Recombinant mouse FcγRI/CD64 (R&D Systems,
cat# 2074-FC-050) with a C-terminal 6xHis-tag constituted at 500 μg
mL–1 in sterile PBS was added to NTA(Ni)-terminated
QDs for 30 min at RT. The concentration of the 6xHis-tag Fc-receptor
was calculated to give three Fc-receptors per QD. The resulting solution
was passed through a syringe filter with a 200 nm polytetrafluoroethylene
membrane to remove any aggregates.
Targeting experiments were
performed by conjugating antibodies of four pancreatic cancer biomarkers:57 (link) Claudin-4 (anti-CLDN4, #329400, Invitrogen),
mesothelin (anti-MSLN, #354200, Invitrogen), mucin-4 (anti-MUC4, #354900,
Invitrogen), and cadherin-11 (anti-CDH11, ab151446, Abcam). Antibodies
as supplied (100 μg in 200 μL) were added to a suspension
of the QD-FcR complex to give three antibodies per QD and incubated
for 1 h at RT.
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