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Multina capillary electrophoresis system

Manufactured by Shimadzu
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The MultiNA capillary electrophoresis system is a laboratory instrument designed for the separation and analysis of biomolecules, such as DNA, RNA, and proteins, using capillary electrophoresis technology. The system provides high-resolution separation and precise quantification of target analytes.

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Lab products found in correlation

Premix taq, by Takara Bio (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

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MultiNA (51 citations) MultiNA System (5 citations)

2 protocols using multina capillary electrophoresis system

1

PCR-Based Serotyping of Streptococcus

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Serotypes c, e, f, and k were confirmed via PCR using serotype-specific sets of primers as described previously [11 (link)]. The composition of the PCR mixture was as follows: 5 µL Premix Taq™ (TaKaRa Taq™ Version 2.0), 5 ng DNA, and 5 µM primers, scaled to 10 µL with PCR-grade water. The PCR was performed according to a previously reported method [11 (link)]. PCR products were confirmed using the MultiNA capillary electrophoresis system (Shimadzu Corporation).
Kimijima M., Narisawa N., Hori E., Mandokoro K., Ito T., Ota Y., Sashida M., Kawai Y, & Takenaga F. (2024). Nattokinase, a Subtilisin-like Alkaline-Serine Protease, Reduces Mutacin Activity by Inactivating the Competence-Stimulating Peptide in Streptococcus mutans. Pathogens, 13(4), 286.
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2

Transcriptomics of Human Brain Tissue

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Ten milligrams of snap‐frozen human brain tissue was used for total RNA extraction using Trizol reagent (Thermo Fisher Scientific), followed by DNAse digestion. RNA quality was verified on the Shimadzu MultiNA capillary electrophoresis system (Shimadzu). Following Dynabead Oligo(dT) enrichment (Invitrogen), messenger RNA (mRNA) was prepared into sequence‐ready libraries with the NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). Libraries were sequenced as described earlier with a 100 bp single‐read length. Sequence tags were aligned to the human reference genome hg19 and Ensembl transcript reference (Homo Sapiens 75) using STAR aligner.21 The numbers of read tags aligning to each region were extracted using featureCounts20 producing a matrix of counts (tags per gene per sample). Genes with fewer than 100 sequence reads across all samples were removed from further analysis.
Kobow K., Ziemann M., Kaipananickal H., Khurana I., Mühlebner A., Feucht M., Hainfellner J.A., Czech T., Aronica E., Pieper T., Holthausen H., Kudernatsch M., Hamer H., Kasper B.S., Rössler K., Conti V., Guerrini R., Coras R., Blümcke I., El‐Osta A, & Kaspi A. (2019). Genomic DNA methylation distinguishes subtypes of human focal cortical dysplasia. Epilepsia, 60(6), 1091-1103.
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