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P4359

Manufactured by Merck Group
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P4359 is a laboratory instrument designed for performing spectrophotometric measurements. It is capable of analyzing the absorbance or transmittance of light through samples across a range of wavelengths.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Nunc lab tek 2 cc2 chamber slide, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Acetylated ldl, by Thermo Fisher Scientific (2 mentions) Pifithrin α, by Merck Group (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions) Necrostatin 1, by Merck Group (1 mentions) N9037, by Merck Group (1 mentions)

4 protocols using p4359

1

Acetylated LDL Uptake Assay

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6×104 fibroblasts per cm2 were cultured overnight at 37°C, 5% CO2 on Growth Factor Reduced Matrigel Basement Membrane Matrix (BD) coated wells of Nunc Lab-Tek II CC2 chamber slides (Thermo). Serum starved or unstarved cells were cultured in IMDM, 1X Penicillin/Streptomycin or IMDM, 1X Penicillin/Streptomycin, 10% fetal bovine serum (Gibco), respectively. Pifithrin-α 100μM (P4359, Sigma) or RITA 0.1μM (506149, EMD Chemicals) were added to the cells cultured with the above culture medium. Acetylated LDL (Invitrogen) uptake was performed as described40 (link).
Ubil E., Duan J., Pillai I.C., Rosa-Garrido M., Wu Y., Bargiacchi F., Lu Y., Stanbouly S., Huang J., Rojas M., Vondriska T.M., Stefani E, & Deb A. (2014). Mesenchymal-endothelial-transition contributes to cardiac neovascularization. Nature, 514(7524), 585-590.
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2

Transfection of HEK293T Cells with Lipofectamine 2000

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HEK293T cells were obtained from ATCC with verification of cell line identification and mycoplasma negative results. They were expanded using 10% fetal bovine serum (FBS) in high-glucose Dulbecco's modified Eagle's medium (DMEM) with glutamax passaging at a typical rate of 1:100 and maintained at 37°C with 5% CO2. Transfection was conducted using Lipofectamine 2000 (Thermofisher Catalogue # 11668019) using the protocol recommended by the manufacturer with slight modifications outlined below. Twenty-four hours before transfection ∼1.0 × 105 cells were seeded per well in a 12-well plate along with 1 ml of media. A total of 2 μg of DNA and 2 μl of Lipofectamine 2000 were used per well. For Cas9 plasmids, the DNA content per well was 1 μg of pCas9_GFP mixed with 1 μg of gRNA-expressing plasmid. For BE plasmids, 1.5 μg of BE was mixed with 0.5 μg of gRNA plasmid. In the dBE versus nBE comparison used to generate Figure 4, Pifithrin-α (10 ng/μl) from Sigma-Aldrich P4359 (source # 063M4741V, Batch # 0000003019) and bFGF from Thermo Scientific (catalog # 13256029) was added to the media 30 min before transfection and maintained in the first day media change.
Smith C.J., Castanon O., Said K., Volf V., Khoshakhlagh P., Hornick A., Ferreira R., Wu C.T., Güell M., Garg S., Ng A.H., Myllykallio H, & Church G.M. (2020). Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Research, 48(9), 5183-5195.
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3

Acetylated LDL Uptake Assay

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6×104 fibroblasts per cm2 were cultured overnight at 37°C, 5% CO2 on Growth Factor Reduced Matrigel Basement Membrane Matrix (BD) coated wells of Nunc Lab-Tek II CC2 chamber slides (Thermo). Serum starved or unstarved cells were cultured in IMDM, 1X Penicillin/Streptomycin or IMDM, 1X Penicillin/Streptomycin, 10% fetal bovine serum (Gibco), respectively. Pifithrin-α 100μM (P4359, Sigma) or RITA 0.1μM (506149, EMD Chemicals) were added to the cells cultured with the above culture medium. Acetylated LDL (Invitrogen) uptake was performed as described40 (link).
Ubil E., Duan J., Pillai I.C., Rosa-Garrido M., Wu Y., Bargiacchi F., Lu Y., Stanbouly S., Huang J., Rojas M., Vondriska T.M., Stefani E, & Deb A. (2014). Mesenchymal-endothelial-transition contributes to cardiac neovascularization. Nature, 514(7524), 585-590.
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4

Modulating Cell Signaling in Lung Cancer

Cited 1 time
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When lung cancer cell lines A549 and H1299 reached 50–70% confluence, they were transfected with the B2 plasmid with PEI for 4 h. Then, the culture medium was changed and they were incubated for another 48 h. In inhibitor experiments, cells were treated with a P53 inhibitor (30 μM Pifithrin-α [52 (link), 53 (link)], Sigma Aldrich, P4359) or a necroptosis inhibitor (40 μM necrostatin-1 [54 (link)], Sigma Aldrich, N9037).
Chiu H.W., Su Y.C, & Hong J.R. (2017). Betanodavirus B2 protein triggers apoptosis and necroptosis in lung cancer cells that suppresses autophagy. Oncotarget, 8(55), 94129-94141.
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