Rabbit anti parp1 antibody

Co-immunoprecipitation samples were subjected to SDS-PAGE on acrylamide gel. For Commassie blue staining, the acrylamide gel was stained with Brilliant Blue R250 Protein solution (Elpis, Korea) for 1 h with gentle agitation and then washed three times with destaining solution containing 20% methanol and 10% acetic acid for 1 h. For Western blotting, SDS-PAGE gel was transferred onto PVDF membranes (Millipore). After blocking with 5% skimmed milk in TBS-T buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween20) for 1 h, membranes were incubated with the primary antibodies at 4°C with shaking overnight. After incubated with the secondary antibody for 2 h, immunocomplexes were detected by use of the femto, Pico or ECL reagent (Thermo). Antibodies for Western blotting are from the companies as follows: mouse/rabbit anti-flag antibody (Sigma), mouse anti-VRK1 (Santa Cruz), rabbit anti-VRK3 (Sigma), mouse ant-ALY antibody (Santa Cruz), rabbit anti-hsp70 (Origene), rabbit anti-XRCC5 (Santa Cruz), rabbit anti-B23 antibody (Santa Cruz), rabbit anti-C23 antibody (Santa Cruz), rabbit anti-PARP1 antibody (Cell signaling), rabbit anti-cyclin B1 (Santa Cruz), rabbit anti-p53 (Cell signaling) and rabbit anti-BAF [previously used (Kim et al., 2015 (link))].