For LC–MS/MS, a Varian HPLC–MS/MS system 1200L triple–quadrupole mass spectrometer equipped with an electrospray ionization source was used. Analytes were separated in incubation mixtures by HPLC with a Luna C18, 5 μm, 50 mm × 2.1 mm column (Phenomenex) or a Acquity BEH C18, 50*2.1mm, 1,7μm column (Waters). The mobile phase solvents used were 0.1% formic acid in water (A) or 0.1% formic acid in acetonitrile (B) using a gradient: 2–98% B for 2.30 min; hold at 98% B for 1.00 min; 98–2% B for 0.10 min; 2% B hold for 1.50 min. The injection volume was 10 μL, and the flow rate was 0.6 mL·min−1. MS analysis were performed with MRM detection using the parameters optimized for each compounds, with MS Workstation software (version 6.3.0 or higher).
Hplc ms ms system 1200l triple quadrupole mass spectrometer
Manufactured by Agilent Technologies
The HPLC–MS/MS system 1200L triple–quadrupole mass spectrometer is a high-performance liquid chromatography tandem mass spectrometry instrument. It combines high-pressure liquid chromatography with a triple-quadrupole mass spectrometer for sensitive and selective detection and quantification of target analytes in complex samples.
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Lab products found in correlation
2 protocols using hplc ms ms system 1200l triple quadrupole mass spectrometer
1
LC-MS/MS Analysis of Biomolecules
2
LC-MS/MS Analytical Protocol for Compound Separation
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For LC−MS/MS, a Varian HPLC−MS/MS system 1200L triple−quadrupole mass spectrometer equipped with an electrospray ionization source was used. Analytes were separated in incubation mixtures by HPLC with a Luna C18, 5 μm, 50 mm × 2.1 mm column (Phenomenex) or a Acquity BEH C18, 50 × 2.1 mm, 1, 7 μm column (Waters). The mobile phase solvents used were 0.1% formic acid in water (A) or 0.1% formic acid in acetonitrile (B) using a gradient: 2–98% B for 2.30 min; hold at 98% B for 1.00 min; 98–2% B for 0.10 min; 2% B hold for 1.50 min. The injection volume was 10 μl, and the flow rate was 0.6 ml min−1. MS analysis were performed with MRM detection using the parameters optimized for each compounds, with MS Workstation software (version 6.3.0 or higher).
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