The lactate amount was measured in cell culture media after 24 h of treatment with K-LATE kit (Megazyme, Bray, Ireland) according to the data sheet provided by the manufacturer. Lactate content was normalized on the protein content of the same sample.
K late kit
Manufactured by Megazyme
Sourced in Ireland
The K-LATE kit is a laboratory test kit designed for the quantitative determination of lactate in various sample types. The kit provides the necessary reagents and protocols to perform the analysis.
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Lab products found in correlation
4 protocols using k late kit
1
Lactate Quantification in Cell Cultures
2
Lactate Quantification Assay
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Lactate amount was assayed in cells medium with K-LATE kit (Megazyme) according manufacturer's instructions. Lactate content was normalized on protein content of the same sample.
3
Cellular Metabolic Profiling Assay
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The biochemical analysis was performed in the same experimental condition of the proteomics studies. The Oxygen Consumption Rate (OCR) analysis was performed using a Clark-type O2 electrode inserted in a thermostatic airtight chamber (Oxygraph Plus System, Hansatech Instruments). Briefly, control and treated cells were trypsinized, washed with PBS, and suspended in a culture medium at the concentration of 106 cells/mL. To measure the steady-state OCR,1 ml of cell suspension was transferred to the airtight chamber maintained at 37 °C, and oxygen consumption was monitored for 10 min. The OCR values were normalized from the total protein of each sample (nmol of consumed O2/mg of protein) and reported as the percentage of OCR relatively to control cells.
Lactate amounts of cell culture media coming from untreated and treated cells were measured with the K-LATE kit (Megazyme) according to the datasheet provided by the manufacturer. Lactate content was normalized to the total protein of each sample (mmoles of produced lactate/mg of protein) and reported as the percentage of lactate production with respect to untreated cells.
Lactate amounts of cell culture media coming from untreated and treated cells were measured with the K-LATE kit (Megazyme) according to the datasheet provided by the manufacturer. Lactate content was normalized to the total protein of each sample (mmoles of produced lactate/mg of protein) and reported as the percentage of lactate production with respect to untreated cells.
4
Lactate Production in GAS Cultures
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GAS was cultured in 96-well plates as described earlier for growth analysis. After 24 h of growth, cultures were centrifuged (5000 ×g, 4°C, 10 min) and concentrations of lactate in the supernatants were determined using K-LATE kit (Megazyme).
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