Creatinine elisa kit

Samples from the rat’s blood were collected for serum separation. Serum samples were used to measure the levels of creatinine (Creatinine ELISA Kit, Catalog #: E4370, BioVision, Inc., USA), BUN (BUN ELISA Kit, Catalog #: MBS2611085, MyBioSource, USA), estrogen (Estrogen ELISA Kit, Catalog #: K4266, BioVision, Inc., USA) and progesterone (Progesterone ELISA Kit, Catalog #: K7416, BioVision, Inc., USA), this was performed by commercial kits and according to the kit’s recommendations. Moreover, kidney samples were collected immediately after sacrifice, washed twice in warm PBS, and then subjected to homogenization, as we previously reported [33 (link)]. The antioxidant markers evaluated in kidney tissue were the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT); their measurement was conducted using commercial kits and following the kit’s recommendations (Bio-diagnostics Co., Cairo, Egypt and BioVision, Inc., Milpitas, CA, USA).

Kidney tissues were homogenized in RIPA lysis buffer (Thermo Scientific, Pittsburgh, PA, USA). All samples diluted tenfold were quantified using the NanoDrop 2000 (Thermo Scientific). Mouse AREG ELISA kits (R&D Systems) were used according to the manufacturer’s protocol. BUN and creatinine were analyzed using a BUN kit (ARBOR ASSAYS, Ann Arbor, MI, USA) and a creatinine ELISA kit (BioVision, Milpitas, CA, USA), respectively. All ELISA procedures were performed according to the manufacturer’s instructions.