Rabbit anti h3k4me1

Nuclear extract was isolated using Nuclear Extraction Kit (Abcam, cat. no. ab113474) according to the manufacturer’s protocol. Samples and pre-stained protein markers were electrophoresed through 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels, and then transferred to polyvinylidene fluoride (PVDF) membranes, using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad). The membrane was then incubated overnight at 4 ℃ with primary antibodies, rabbit anti-H3K27ac (1:2000, Active Motif, Carlsbad, CA), rabbit anti-H3K4me1 (1:1000, Cell Signaling, Beverly, MA), or mouse anti-Lamin A/C (1:1000, Sigma), was diluted in 1 × phosphate-buffered saline with Tween (PBST). The membranes were incubated at room temperature for 2 h, with secondary antibodies (Thermo Fisher, anti-mouse, 1:1000 or anti-rabbit 1:1000, diluted in 1 × PBST). Proteins were detected using an enhanced chemiluminescence horseradish peroxidase (HRP) substrate detection kit (Merck Millipore) by BioSpectrum 2D Imaging System (UVP, BioSpectrum 800).

UNC-0638 (cat no U4885) was from Sigma-Aldrich (Steinheim, Germany), UNC-1999 (cat no S7165) from Selleckchem (Houston, TX), H-Leu-Leu-OMe (LLME) (cat no 4000725.0005) from Bachem (Bubendorf, Switzerland). Nafamostat mesylate (cat no N0289), staurosporine (cat no S4400), mefloquine (cat no M2319), Pefabloc SC (cat no 11429868001), pepstatin A (cat no 516481) and E-64d (cat no E8640) were from Sigma-Aldrich (Steinheim, Germany). Rabbit anti-H3K9me2 antibody (cat no 07-441) was from EMD-Millipore (Darmstadt, Germany). Rabbit anti-H3K4me1 (cat no 5323S) monoclonal antibody was from Cell Signaling Technology (Danvers, MA). Rabbit anti-histone 2A (H2A) (cat no ab177308), H2B (cat no ab1790), H3 (cat no ab1791) and H4 (cat no ab177840) antibodies, mouse anti-H3K27me3 (cat no ab6002) monoclonal antibody and rabbit anti-H3S10p (cat no ab272166) polyclonal antibody were from Abcam (Cambridge, UK). A mouse monoclonal antibody to β-actin (cat no sc-517582) was from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit anti-H2BS14p polyclonal antibody (cat no PA5-105775) was from Invitrogen (Eugene, OR). Rabbit anti-H2BK16ac (cat no 39121) was from Active Motif (Carlsbad, CA).