Reactive red 120

Manufactured by Merck Group

Sourced in United States

Reactive Red 120 is a reactive dye commonly used in laboratory applications. It is a water-soluble, reddish-brown powder that can be used for various purposes, such as staining and labeling. The core function of Reactive Red 120 is to provide a stable, colorful dye for various experimental and analytical procedures.

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Reactive RED 120-agarose (3 citations)

7 protocols using reactive red 120

Decolorization of Landfill Leachate

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2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 1-aminobeznotriazole (ABT) were obtained from Sigma-Aldrich (Germany). Stock solutions of ABT were prepared in ethanol at a concentration of 50 mM.
o-tolidine was purchased from Chempur (Poland). A stock solution of o-tolidine (10 mg mL^-1) was prepared in ethanol. Liquid chromatography solvents were obtained from Avantor Performance Materials (Poland).
The azo dyes Reactive Orange 16 (CAS 12225–83-1), Acid Red 27 (CAS 915–67-3), Acid Orange 7 (CAS 633–96-5), Reactive Red 120 (CAS 61951–82-4), and Reactive Black 5 (CAS 17095–24-8) were purchased from Sigma-Aldrich (Germany). Stock solutions were prepared in water at a concentration of 50 mg mL^-1.
Cd(NO₃)₂ × 4 H₂O, K₂Cr₂O₇, and ZnSO₄ × 7 H₂O were purchased from Sigma-Aldrich (Germany). Metal stock solutions were prepared by dissolving in deionized water at a concentration of 1 M.
Samples of landfill leachate (L1 and L2) collected from the landfill of the former “Boruta” Dye Industry Plant in Zgierz, Poland (Fig. 1) were kindly supported by the Voivodeship Inspectorate of Environmental Protection in Łódź, Poland.

Góralczyk-Bińkowska A., Długoński A., Bernat P., Długoński J, & Jasińska A. (2021). Environmental and molecular approach to dye industry waste degradation by the ascomycete fungus Nectriella pironii. Scientific Reports, 11, 23829.

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Cultivation and Expression of Saccharomonospora viridis

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Saccharomonospora viridis DSM 43017 was obtained from the China General Microbiological Culture Collection Center, Beijing (reference number CGMCC 4.1324). The strain was cultivated in a shaker flask at 45°C in STS medium (1.0% (w/v) soy peptone, 1.0% (w/v) glucose, 0.2% (w/v) yeast extract, 0.2% (w/v) NaCl, and 0.2% casein enzymatic hydrolysate, all from Biodee, Beijing, China), adjusted pH to 8.0 with NaOH prior to autoclaving.
Escherichia coli DH5α and E. coli BL21 (Tiangen, Beijing, China) (were cultivated in Luria Bertani (LB) medium at 37°C for gene cloning, sequencing, and expression. The pEASY-T3 vector (TransGen, Beijing, China) was used for plasmid gene cloning and sequencing. The plasmid pET-28a (+) (Takara Bio, Otsu, Japan) was used as an expression vector. Manganese peroxidase (MnP), azure B, brilliant green, reactive blue 19, reactive green 19, reactive yellow 2, reactive black 5, reactive red 120, malachite green and crystal violet were purchased from Sigma (St. Louis, MO, USA). All other reagents used were of analytical grade unless otherwise stated.

Yu W., Liu W., Huang H., Zheng F., Wang X., Wu Y., Li K., Xie X, & Jin Y. (2014). Application of a Novel Alkali-Tolerant Thermostable DyP-Type Peroxidase from Saccharomonospora viridis DSM 43017 in Biobleaching of Eucalyptus Kraft Pulp. PLoS ONE, 9(10), e110319.

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Diffusional Properties of Gradient Porosity Silk Catheters

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A two-compartment testing chamber was 3D printed to assess the diffusional properties of the gradient porosity silk catheters. The chambers were printed with acrylonitrile butadiene styrene (ABS) plastic. Chamber dimensions were 56 mm length × 20 mm wide × 20 mm depth, with two chambers each 26 mm length × 16 mm wide × 18 mm depth, separated by a 2 mm thick wall with a 1.5 mm diameter channel extending through the bifurcation. The non-porous ends of the dip-coated tubes were inserted into the opening of an ALZET (Cupertino, CA) osmotic pump, prefilled with 100 μL of Reactive Red 120 (Sigma-Aldrich, St. Louis, MO) solution. Tubes were inserted through the aperture on the impermeable septum separating the two compartments, such that the overlapping porous/non-porous region was centered in the middle of the aperture. Silicone caulking agent was used to seal the edges of the bifurcation channel to prevent flow between the chambers. Each chamber was filled with 5 mL of 1xPBS solution. Chambers and tubes were stored at room temperature on a shaker plate set at the lowest setting to induce agitation. Dye release was measured at daily time points over two weeks using a spectrophotometric analysis. Absorbance measurements at 540 nm wavelength light were recorded as a means of quantifying the release of dye over time.

Brown J.E., Tozzi L., Schilling B., Kelmendi-Doko A., Truong A.B., Rodriguez M.J., Gil E.S., Sucsy R., Valentin J.E., Philips B.J., Marra K.G., Rubin J.P, & Kaplan D.L. (2018). Biodegradable Silk Catheters for the Delivery of Therapeutics across Anatomical Repair Sites. Journal of biomedical materials research. Part B, Applied biomaterials, 107(3), 501-510.

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Dye Decolorization by Bacteria

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The Reactive Red 120 (RR120) used in this research was purchased from Sigma, Aldrich, CO, USA. Nutrient agar, yeast extracts and nutrient broth were obtained from Friedemann Schmidt Shd. Bhd. Malaysia. Tris and acetate buffers were obtained from Merck KGaA, Germany. Meanwhile, other chemicals used in this study were obtained from Fisher Malaysia.

Manogaran M., Yasid N.A., Othman A.R., Gunasekaran B., Halmi M.I, & Shukor M.Y. (2021). Biodecolourisation of Reactive Red 120 as a Sole Carbon Source by a Bacterial Consortium—Toxicity Assessment and Statistical Optimisation. International Journal of Environmental Research and Public Health, 18(5), 2424.

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Extraction and Characterization of Eriobotyra japonica Leaves

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Eriobotyra japonica (Thunb.) leaves were collected from Yangshan County, Guangdong Province, China (23°25′21.74″ N 113°03′24.25″ E). The leaves were washed several times with distilled water, shade dried for 3 days in the air, and then crushed into a good powder using the electronic blender. Thirty grams of the leaf powder was stirred with 500 mL of Milli-Q water and kept at 65 °C for 0.5 h. Then the extracts were filtered by using Whatman No. 1 filter paper after cooling to room temperature. The extract was stored at 4 °C for future use. Silver nitrate (AgNO₃, 99.8%) and sodium borohydride (NaBH₄, 98%) were procured from Aladdin Industrial Corporation (Shanghai, China). Reactive Red 120 and Reactive Black 5 were purchased from Sigma-Aldrich (Shanghai, China).

Yu C., Tang J., Liu X., Ren X., Zhen M, & Wang L. (2019). Green Biosynthesis of Silver Nanoparticles Using Eriobotrya japonica (Thunb.) Leaf Extract for Reductive Catalysis. Materials, 12(1), 189.

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Microbial Decolorization of Textile Azo Dyes

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The textile azo dyes Remazol Brilliant Violet 5R (RBV 5R) and Reactive Red 120 (RR 120) were procured from SIGMA, India. The minimal salt media (MSM) used for enrichment and decolorization was prepared by adding the following components: Na2HPO4 (12.8 g/L), KH2PO4 (3 g/L), NH4Cl (1 g/L), NaCl (0.5 g/L), 0.05 M MgSO4 (10 mL/L), 0.01 M CaCl2 (10 mL/L) and 20% glucose (30 mL/L) (Lalnunhlimi and Krishnaswamy, 2016) . All the chemicals used in the study were of high purity and analytical grade procured from Hi-Media Laboratories, Mumbai. Furthermore, nutrient broth and nutrient agar media (Hi-Media Laboratories) were used for culture maintenance.

Ramaswamy R., Sundaravadivel K., Prakash S., & Mohan A. (2021). Biodecolorization of azo dye mixture (Remazol Brilliant Violet 5R and Reactive Red 120) by indigenous bacterial consortium isolated from dye contaminated soil. Malaysian Journal of Microbiology.

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Purification and Characterization of Phthalate Degrading Enzymes

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All steps were carried out under anoxic conditions (95% N 2 , 5% H 2 ). Around 3 g of phthalate grown cells (wet mass) were suspended in 9-12 ml buffer A and lysed using a French pressure cell. After ultracentrifugation, the supernatant was filtered through a 0.2-μm sterile filter (Filtropur S 0.2, Sarstedt) and applied to a 12 ml DEAE-Sepharose column (GE Healthcare), equilibrated with buffer A at a flow rate of 0.5 ml min -1 . The column was washed with two bed volumes of buffer A and with buffer A supplemented with 15 mM KCl (PCD) or 20 mM KCl (PCL). Fractions eluting in buffer A at higher KCl concentrations as indicated in the results section were tested for PCL/PCD activities. Activity containing fractions were concentrated and used for SDS-PAGE analysis and enzymatic assays. Other chromatography materials tested for PCL/PCD enrichment were Resource Q-Sepharose anionic exchanger, Superdex 200 10/300 Gl gel filtration (all GE Healthcare), and the affinity dyes Reactive Green 5 Agarose, Reactive Red 120, and Cibacron Blue 3GA (1 ml columns each, all Sigma-Aldrich). All columns were equilibrated with buffer A, and elution was by varying KCl, or in case of the affinity dyes, phthalate concentrations (0.1-1 M).

Geiger R.A., Junghare M., Mergelsberg M., Ebenau-Jehle C., Jesenofsky V.J., Jehmlich N., von Bergen M., Schink B, & Boll M. (2019). Enzymes involved in phthalate degradation in sulphate-reducing bacteria. Environmental microbiology, 21(10).

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