Fli 1 polyclonal antibody

Manufactured by Cell Signaling Technology

The Fli-1 polyclonal antibody is a laboratory reagent used to detect the presence and localization of the Fli-1 protein in biological samples. Fli-1 is a transcription factor that plays a role in various cellular processes. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and distribution of Fli-1 in different cell types and tissues.

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Lab products found in correlation

Odyssey imaging system, by LI COR (2 mentions) Antibody to β actin, by Cell Signaling Technology (2 mentions) Iblot 2 transfer stack, by Thermo Fisher Scientific (1 mentions) Criterion tgx stain free protein gel, by Bio-Rad (1 mentions) Criterion tgx precast gel, by Bio-Rad (1 mentions) Pvdf membrane, by Thermo Fisher Scientific (1 mentions)

2 protocols using fli 1 polyclonal antibody

Protein Extraction and Western Blot Analysis

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Cells were lysed in the Radioimmunoprecipitation assay (RIPA) buffer as previously described (33 ). After lysis, the supernatant was collected. Equal amounts of protein (20 μg-40 μg) were run on for 1.5 hours at 130V using 4–20% Criterion TGX Stain-Free Protein Gels (Bio-Rad, Hercules, CA) and electro-transferred to a PVDF membrane by Iblot2 transfer stacks (Invitrogen, Waltham, MA). Transferred proteins were probed with both a Fli-1 polyclonal antibody described previously (30 (link)) and an antibody to β-actin (Cell Signaling, Beverly, MA). The results were visualized using the Odyssey Imaging System (LI-COR, Lincoln, NE).

Wang X., Richard M.L., Li P., Henry B., Schutt S., Yu X.Z., Fan H., Zhang W., Gilkeson G, & Zhang X.K. (2020). Expression of granulocyte-macrophage colony-stimulating factor is regulated by Fli-1 transcription factor, a potential drug target. Journal of immunology (Baltimore, Md. : 1950), 206(1), 59-66.

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Western Blot Analysis of Fli-1 Protein

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Cell lysates obtained during the luciferase assay were subsequently run on a 4–15% Criterion TGX Precast gel (Bio-Rad, Hercules, CA) for two hours at 120V. The proteins were then transferred to a PVDF membrane (Life Technologies) and probed with a Fli-1 polyclonal antibody described previously (33 (link)) and an antibody to β-actin (Cell Signaling, Beverly, MA). The results were analyzed on an Odyssey Imaging System (LI-COR, Lincoln, NE).

Richard M.L., Brandon D., Nowling T.K., Watson D.K, & Zhang X.K. (2014). Fli-1 controls transcription from the MCP-1 gene promoter, which may provide a novel mechanism for chemokine and cytokine activation. Molecular immunology, 63(2), 566-573.

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