Sc 18917

Manufactured by Santa Cruz Biotechnology

Sc-18917 is a laboratory instrument manufactured by Santa Cruz Biotechnology. It is designed for general laboratory use. The core function of this product is to perform basic laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Sc 56, by Santa Cruz Biotechnology (2 mentions) Dxm1200f, by Nikon (2 mentions) Eclipse e100 microscope, by Nikon (1 mentions) Axioplan, by Nikon (1 mentions) Tunel label mix, by Roche (1 mentions) Axioplan microscope, by Nikon (1 mentions) Ab8158, by Abcam (1 mentions) Sc 74528, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sc 18917

Immunohistochemical Analysis of Tumor Samples

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Tumors were fixed and 5-μm sections were prepared following standard procedures. The antibodies used were mAb anti-PCNA antibody (PC10) (1:200, sc-56, Santa Cruz) and anti-CD34 (1:100, sc-18917, Santa Cruz). The slices were examined with a Zeiss Axioplan microscope equipped with a Nikon DXM1200F digital camera. PCNA index was quantified in four randomly selected fields from each animal, and CD34 positive areas analyzed using ImageJ software. Apoptotic cells with fragmented nuclei were detected in paraffin samples using TUNEL labeling containing fluorescein-dUTP and -dNTPs (TUNEL Label Mix, Roche). TUNEL positive cells were observed and quantified using a NIKON Eclipse E-100 microscope.

Stefanovic M., Tutusaus A., Martinez-Nieto G.A., Bárcena C., de Gregorio E., Moutinho C., Barbero-Camps E., Villanueva A., Colell A., Marí M., García-Ruiz C., Fernandez-Checa J.C, & Morales A. (2016). Targeting glucosylceramide synthase upregulation reverts sorafenib resistance in experimental hepatocellular carcinoma. Oncotarget, 7(7), 8253-8267.

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Liver Histology and Immunohistochemistry

Cited 2 times

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Livers were fixed and 7-μm sections were routinely stained with H&E or paraffin sections (5-μm) were prepared following standard procedures22 (link)23 (link)26 (link). The antibodies used were rat mAb anti-CD34 (1:50 dilution, ab8158, Abcam), goat polyclonal anti-antiogenin (C1) (1:200, sc-74528, Santa Cruz Biotechnology), mAb anti-PCNA antibody (PC10) (1:200 dilution, sc-56, Santa Cruz Biotechnology) and anti-CD34 (1:100 dilution, sc-18917, Santa Cruz Biotechnology). The slices were examined with a Zeiss Axioplan microscope equipped with a Nikon DXM1200F digital camera. PCNA index was quantified in four randomly selected fields from each animal, and CD34 positive areas analyzed using ImageJ software.

Bárcena C., Stefanovic M., Tutusaus A., Martinez-Nieto G.A., Martinez L., García-Ruiz C., de Mingo A., Caballeria J., Fernandez-Checa J.C., Marí M, & Morales A. (2015). Angiogenin Secretion From Hepatoma Cells Activates Hepatic Stellate Cells To Amplify A Self-Sustained Cycle Promoting Liver Cancer. Scientific Reports, 5, 7916.

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