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Cytomics fc500 flow cytometer

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The BD CYTOMICS FC500 Flow Cytometer is a laboratory instrument used for the analysis and quantification of particles, cells, and cellular components in a fluid sample. It utilizes laser technology and detectors to measure the size, granularity, and fluorescence properties of individual particles as they pass through a focused light beam.

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Lab products found in correlation

Nocodazole, by Merck Group (2 mentions) Aphidicolin, by Merck Group (2 mentions)

Spelling variants of this product

FC500 flow cytometer (30 citations) FC500 (16 citations) Cytomics FC500 (5 citations) FC500 cytometer (2 citations)

3 protocols using cytomics fc500 flow cytometer

1

Cell Cycle Analysis after TMZ and Radiation

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Cells were treated with 10 µM TMZ for 48 hours or with 10 Gy of ionizing radiation (cesium source; JL Shepherd and Associates), and fixed at the specified time points in ethanol overnight at −20°C. Cell cycle stage was assessed by single-parameter flow cytometry (after propidium iodide staining for DNA content) using a BD CYTOMICS FC500 Flow Cytometer (Becton Dickinson), as described before (32 (link)).
Gil del Alcazar C.R., Todorova P.K., Habib A.A., Mukherjee B, & Burma S. (2016). Augmented HR Repair Mediates Acquired-temozolomide Resistance in Glioblastoma. Molecular cancer research : MCR, 14(10), 928-940.
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2

Synchronizing HEK-293 cells for cell cycle analysis

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HEK-293 cells were synchronized in G1 by treatment with aphidicolin (2μg/ml, Sigma) for 16 hours and then released into regular media for either 5 hours (S phase) or 10 hours (G2 phase)19 (link). Alternatively, HEK-293 cells were synchronized in late G2 by treatment with nocodazole (100ng/ml, Sigma) for 16 hours. Cell cycle stage was determined by flow cytometry using a BD CYTOMICS FC500 Flow Cytometer (Becton, Dickinson and Company).
Tomimatsu N., Mukherjee B., Hardebeck M.C., Ilcheva M., Camacho C.V., Harris J.L., Porteus M., Llorente B., Khanna K.K, & Burma S. (2014). Phosphorylation of EXO1 by CDKs 1 and 2 regulates DNA end resection and repair pathway choice. Nature communications, 5, 3561.
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3

Synchronizing HEK-293 cells for cell cycle analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK-293 cells were synchronized in G1 by treatment with aphidicolin (2μg/ml, Sigma) for 16 hours and then released into regular media for either 5 hours (S phase) or 10 hours (G2 phase)19 (link). Alternatively, HEK-293 cells were synchronized in late G2 by treatment with nocodazole (100ng/ml, Sigma) for 16 hours. Cell cycle stage was determined by flow cytometry using a BD CYTOMICS FC500 Flow Cytometer (Becton, Dickinson and Company).
Tomimatsu N., Mukherjee B., Hardebeck M.C., Ilcheva M., Camacho C.V., Harris J.L., Porteus M., Llorente B., Khanna K.K, & Burma S. (2014). Phosphorylation of EXO1 by CDKs 1 and 2 regulates DNA end resection and repair pathway choice. Nature communications, 5, 3561.
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