Diatomaceous earth (Celite® 209), Sodium Carboxymethyl cellulose (Na-CMC), Silver nitrate (AgNO3) and L-Ascorbic acid (L-AA) were purchased from Sigma Aldrich, St. Louis, MO, USA. Stannous chloride (SnCl2) and Hydrochloride acid (HCl) were procured from Alfa Aesar. The biotoxin standard, Domoic Acid was purchased from MuseChem, Arrakis Tek Inc., Fairfield, NJ, USA. Dungeness crab was brought from seafood market in Newport, situated along Oregon’s central coast. All the solutions and dilutions were prepared in ultrapure Milli Q (18.2 MΩ cm) until mentioned otherwise.
L ascorbic acid l aa
Manufactured by Merck Group
Sourced in United States
L-Ascorbic acid (L-AA) is a chemical compound used in various laboratory applications. It is a form of vitamin C and serves as an antioxidant. L-AA is commonly used as a reagent in analytical and research procedures.
Automatically generated - may contain errors
Lab products found in correlation
H3po4, by Merck Group (2 mentions)
Graphite flake, by Thermo Fisher Scientific (2 mentions)
Sodium nitrate nano3, by Merck Group (2 mentions)
Silver nitrate agno3, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
L lactic acid, by Merck Group (1 mentions)
Matrigel, by Corning (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Chir99021, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Copper chloride, by Merck Group (1 mentions)
K2co3, by Merck Group (1 mentions)
Polyvinyl alcohol pva mw 89 000 98 000, by Merck Group (1 mentions)
Resorcinol, by Merck Group (1 mentions)
Pvp k90, by Merck Group (1 mentions)
Whatman, by Cytiva (1 mentions)
Beta glycerophosphate β gp, by Merck Group (1 mentions)
Oleylamine, by Thermo Fisher Scientific (1 mentions)
16 protocols using l ascorbic acid l aa
1
Domoic Acid Detection in Crab
2
Cardiac Differentiation of hiPSCs
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Human induced pluripotent stem cells (hiPSCs) were cultured in a chemically defined E8 medium (#1014500, Cellapy, China) on Matrigel (1:300, #356231, Corning, Tewksbury MA, USA). hiPSCs underwent differentiation towards cardiomyocytes when they reach ≈90% confluence. From the start of differentiation (D0), the E8 media is switched to cardiac differentiation media CDM3 comprised RPMI1640 (#11875500, Gibco, USA), bovine serum albumin(BSA, #9048‐46‐8, Sigma, USA), and L‐ascorbic acid (LAA, # 1713265‐25‐8, Sigma, USA). During the first two days, the cells were treated with the Gsk3β inhibitor CHIR99021(#252917‐06‐9, Sigma, USA) to potentiate the Wnt signaling pathway. After 48 h (D2), the cells were treated with a Wnt inhibitor C59 (#S7037, Selleck, USA) for consecutive two days. Cell beating could be seen around D8‐D9. Cardiomyocytes underwent selection and purification metabolically using CDM‐L medium comprised of glucose‐free RPMI‐1640 (#11879‐020, Gibco. USA), BSA, LAA, and L‐lactic acid (#L4263, Sigma, USA). After purification, cardiomyocytes were maintained in CDM3 medium and seeded onto a glass coverslip or into the PLA scaffold, which were pre‐coated with Matrigel at D18‐20. RPMI 1640 medium supplied with 10% FBS was used for the first 24 h culture and then the medium was changed to CDM3 for the consecutive culture. The medium was changed every other day.
3
Curcumin-Loaded Polymeric Nanoformulation
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PEO (Avg Mn 80,000), EC
(48% ethoxy, 4 cP), PVP (Avg Mn 360,000 Da), curcumin (>98.0%,
HPLC),l -ascorbic acid (L-AA), and 2,2-diphenyl-1-picrylhydrazyl
(DPPH)
were purchased from Sigma-Aldrich (South Korea). Hydrogenated castor
oil PEG 40 was purchased from biOriginds Co., Ltd. (UK). Ethanol (EtOH,
≥99.8% AnalaR NORMAPUR ACS, Reag. Ph. Eur. analytical reagent)
was purchased from VWR International (USA). Phosphate saline buffer
(pH 5.5) pellets were purchased from Millipore Sigma (USA). All other
chemicals used were of analytical grade, and water was doubly distilled
before use.
(48% ethoxy, 4 cP), PVP (Avg Mn 360,000 Da), curcumin (>98.0%,
HPLC),
(DPPH)
were purchased from Sigma-Aldrich (South Korea). Hydrogenated castor
oil PEG 40 was purchased from biOriginds Co., Ltd. (UK). Ethanol (EtOH,
≥99.8% AnalaR NORMAPUR ACS, Reag. Ph. Eur. analytical reagent)
was purchased from VWR International (USA). Phosphate saline buffer
(pH 5.5) pellets were purchased from Millipore Sigma (USA). All other
chemicals used were of analytical grade, and water was doubly distilled
before use.
4
Carbon Fabric-Based Electrochemical Devices
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Woven carbon fabric HexForce® 48,193 plain 12 K, manufactured by Hexcel (Stamford, CT, USA), was used in this work. The separator used was of filter paper (FP) or woven glass fiber (WGF). The glass fiber separator (E-Fiberglass Woven Roving) was supplied by Castro Composites® (Pontevedra, Spain). Glass Filter paper Whatman, Lignin alkaline (low sulfonate content), resorcinol, formaldehyde (37 wt% in H2O), copper acetate dihydrate, copper chloride, (L)-ascorbic acid (L-AA), PVP K90, K2CO3, KOH, H3PO4 (≥85 wt% in H2O), and Poly(vinyl alcohol) (PVA, Mw 89,000–98,000, 99+% hydrolyzed) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Liquid sizing agent (SICIZYL) was purchased from Nanocyl S.A. (Sambreville, Belgium). The CuO seed on carbon cloth was done following the procedure previously reported in the literature [27 (link)]. The carbon aerogel modification was prepared following the procedure reported [28 (link)].
5
Osteoblast Differentiation of MC3T3-E1 Cells
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MC3T3E1 cells were cultured in α-MEM suppled with 10% FBS. For osteoblast differentiation, cells were plated at a density of 1.5 × 105 cells per well in 6-well plate and induced with 50 µg/mL l -ascorbic acid (LAA, Sigma Aldrich, MO, USA) and 10 mM beta-glycerophosphate (β-GP, Sigma Aldrich, MO, USA). Medium was changed every 2 days. Alizarin red staining was performed after differentiated for 18 days.
6
Synthesis of Functional Nanomaterials
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All of the chemicals used in this work, including graphite powder, sodium nitrate (NaNO3), potassium permanganate (KMnO4), hydrogen peroxide (H2O2, 30%), l -ascorbic acid (L-AA, Sigma-Aldrich, Seoul, Korea), molybdenum hexacarbonyl (Mo(CO)6, Sigma-Aldrich), selenium (99.999%, Sigma-Aldrich), oleic acid (OA, 85%, Fluka, Mexico City, Mexico), oleylamine (OAm, 80–90%, Acros Organics, Geel, Belgium), and dodecanethiol were of analytical grade and used as received without further purification.
7
Synthesis and Antibacterial Activity of Graphene Oxide
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Graphite flakes were purchased from Alfa Aesar (Karlsruhe, Germany) (99.8%, 325 mesh), sodium nitrate (NaNO3) was obtained from Merck (Darmstadt, Germany), sulphuric acid (H2SO4, 98%), potassium permanganate (KMnO4), hydrogen peroxide (H2O2, 30 wt % aqueous solution), hydrochloric acid (HCl, 37% aqueous solution) and ammonium hydroxide were acquired from Panreac (Barcelona, Spain), while L-Ascorbic acid (L-AA), poly(vinyl alcohol) (PVA) (Mw= 61,000 Da, degree of hydrolysis 98.0–98.8 mol %), silver nitrate (AgNO3) and phosphate-buffered saline (PBS) were supplied by Sigma–Aldrich (Munich, Germany, and St. Louis, MO, USA). Escherichia coli ATCC 25922 (Gram-negative bacteria) and Staphylococcus aureus ATCC 25923 (Gram-positive bacteria) were obtained from CECT (Spanish Type Culture Collection, Valencia, Spain), and brain heart infusion (BHI) broth, plate count agar (PCA) and Mueller–Hinton broth (MHB) were supplied by Condalab (Madrid, Spain). All chemicals were used as received without further purification.
8
Synthesis of Graphene-Copper Nanocomposites
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Graphite powder (99.99%), copper acetate (Cu(OAc)2·H2O), sodium hydroxide (NaOH), hydrogen peroxide (H2O2, 30% aqueous solution), and hydrochloric acid (HCl) were purchased from Alfa-Aeser Co. sulphuric acid (H2SO4, 98%), sodium nitrate (NaNO3), silver nitrate (AgNO3), ethanol (C2H5OH), l -ascorbic acid (L-AA) and ammonium hydroxide (NH4OH, 25%) are supplied from Sigma-Aldrich chemicals. All chemicals were used without any further purification.
9
Osteoblast Differentiation of MC3T3-E1 Cells
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Pre-osteoblast MC3T3E-1 cells (#CRL-2593, American-type culture collection, Manassas, VA, USA) were cultured at 37 °C in a humidified atmosphere of 5% CO2 and 95% air using α-minimum essential medium (α-MEM) (WELGEME, Inc., Gyeonggido, Korea) without L-ascorbic acid (L-AA) (Sigma-Aldrich, St. Louis, MO, USA) and supplemented with 10% fetal bovine serum and 1 X Gibco ® Antibiotic-Antimycotic (Thermo Fisher Scientific, Waltham, MA, USA), as previously described [48 (link)]. The osteoblast differentiation of MC3T3E-1 cells was induced by changing to an osteogenic supplement medium (OS) containing 50 μg/mL L-ascorbic acid (L-AA) and 10 mM β-glycerophosphate (β-GP), as previously described [48 (link)]. The OS was replaced every 2 days during the incubation period. One hundred percent DMSO was used for the dissolution of SoyB, and the vehicle control was used at a final concentration of 0.1% DMSO.
10
Versatile Synthesis of Graphene Oxide and Graphene Aerogel Composites
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Graphite flakes (average particle size of 7–10 μm, 99% purity, Alfa Aesar), sodium nitrate (NaNO3, 99.0%–100.5%, Merck), sodium hydroxide (NaOH, 98%–100.5%, Sigma-Aldrich), potassium permanganate (KMnO4, 99%), hydrogen peroxide (H2O2, 30%, Merck), and sulfuric acid (H2SO4, 95%–97%, Sigma-Aldrich) were used in the preparation of GOs. L-Ascorbic acid (L-AA, 99%, Sigma Aldrich) was used for the reduction of GOs nanosheets in synthesis GAs. P-Phenylenediamine (p-PDA, 98%, Sigma Aldrich) was used as a monomer for in situ synthesis of p(p-PDA) in the GA networks, and ammonium persulfate (APS, 98%, Sigma Aldrich) was used as an initiator of oxidative p-PDA polymerization. Hydrochloric acid (HCl, 36%–38%, Sigma Aldrich), nitric acid (HNO3, 65%, Sigma Aldrich), sulfuric acid (H2SO4, 98%, Merck), and phosphoric acid (H3PO4, 85%, Sigma Aldrich) were used in the doping of in situ synthesized p(p-PDA) within GAs.
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