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NUP98 is a nuclear pore complex protein that functions as a component of the nuclear pore. It plays a role in mediating bidirectional transport between the nucleus and the cytoplasm.

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Lab products found in correlation

Phospho s6k, by Cell Signaling Technology (1 mentions) Phospho chk1, by Cell Signaling Technology (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg, by Merck Group (1 mentions) Goat anti mouse igg, by Merck Group (1 mentions) Histone h3, by Thermo Fisher Scientific (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Nup153, by Abcam (1 mentions) Lamin a c, by Santa Cruz Biotechnology (1 mentions)

2 protocols using nup98

1

Western Blot Analysis of Signaling Proteins

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Total cell lysates were obtained as described before [14 (link)] and analyzed by SDS-PAGE using primary antibodies against ABL1 (Santa Cruz Biotechnology, sc-56887), phospho-ABL1 (Cell Signaling, 2865), AML1 (Cell Signaling, 4334), NUP98 (Santa Cruz Biotechnology, sc-101546), phospho-CHK1 (Cell Signaling, 2348), CHK1 (Cell Signaling 2360), phospho-CHK2 (Abcam, ab59408), CHK2 (Cell Signaling, 2662), phospho-S6K (Cell Signaling, 9204), S6K (Cell Signaling, 9202), phospho-AKT (Cell Signaling, 9271), AKT (Cell Signaling, 2920), γ-H2AX (Cell Signaling, 2577), histone H3 (Invitrogen, AHO1432) and β-actin (Santa Cruz Biotechnology, sc-47778). The following secondary antibodies conjugated to HRP were used: goat anti-mouse IgG (EMD Millipore, 12-349) and goat anti-rabbit IgG (EMD Millipore, 12-348).
Golovine K., Abalakov G., Lian Z., Chatla S., Karami A., Chitrala K.N., Madzo J., Nieborowska-Skorska M., Huang J, & Skorski T. (2023). ABL1 kinase as a tumor suppressor in AML1-ETO and NUP98-PMX1 leukemias. Blood Cancer Journal, 13(1), 42.
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2

Antibodies Used in MYC Regulation

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The following antibodies were used: MYC (monoclonal anti-pS62 [Abcam, ab78318] and polyclonal anti-pS62 [Zhang et al. 2012 (link)]), anti-pT58 (Abm, Y011034), anti-total (Santa Cruz Biotechnology, N262 and C33), PIN1 (Santa Cruz Biotechnology, sc-15340; Novas Biologicals, 2f2), TPR (Santa Cruz Biotechnology, sc121094 and sc67116), GCN5 (Santa Cruz Biotechnology, sc20698), CDK2 (Santa Cruz Biotechnology, sc-163), CDK4 (Santa Cruz Biotechnology, sc-160), ERK (CSG, 4695S) H3 (Upstate Biotechnology, 31560), H3ac (Millipore, 06-599), NUP153 (Abcam, ab24700), NUP98 (Santa Cruz Biotechnology, sc-74578), NUP214 (Abcam, ab70497), Lamin A/C (Santa Cruz Biotechnology, sc-7292, sc-2068, and sc-6215), V5 (Invitrogen, 1718556), and HA (Abm, G036).
Su Y., Pelz C., Huang T., Torkenczy K., Wang X., Cherry A., Daniel C.J., Liang J., Nan X., Dai M.S., Adey A., Impey S, & Sears R.C. (2018). Post-translational modification localizes MYC to the nuclear pore basket to regulate a subset of target genes involved in cellular responses to environmental signals. Genes & Development, 32(21-22), 1398-1419.
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