Polyclonal or monoclonal antibodies were used to detect the expression of cell-proliferation related proteins, including anti-E2F1 (sc-193; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-Ki-67 (SP6; Abcam, Cambridge, MA, USA). The other antibodies included anti-Cox-2 (c-9897; Cayman Chemicals, Ann Arbor, MI, USA) and anti-cytokeratin-19 (Ab53119; Abcam) as cancer-related makers, and anti-connexin 26 (138100 ; Invitrogen, Carlsbad, CA, USA), anti-connexin 32 (358900; Invitrogen), and anti-connexin 43 (138300; Invitrogen). An antibody against calnexin (BD 610523) used as a control was purchased from Transduction Laboratories (BD Biosciences, San Jose, CA, USA) and used at a 1:1,000 dilution. Anti-mouse, anti-rabbit, and anti-goat IgG antisera conjugated with horseradish peroxidase (HRP) were purchased from DAKO (Glostrup, Denmark).
Anti connexin 26
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-connexin 26 is a lab equipment product used for the detection and identification of the connexin 26 protein. Connexin 26 is a type of gap junction protein found in various tissues, including the skin and inner ear. The anti-connexin 26 product can be used in research and diagnostic applications to study the expression and localization of this protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti ki 67 sp6, by Abcam (1 mentions)
Anti connexin43, by Thermo Fisher Scientific (1 mentions)
Anti e2f1 sc 193, by Santa Cruz Biotechnology (1 mentions)
Anti cytokeratin 19, by Abcam (1 mentions)
Anti cox 2, by Cayman Chemical (1 mentions)
Fitc conjugated cholera toxin b subunit, by Merck Group (1 mentions)
2 protocols using anti connexin 26
1
Immunohistochemical Analysis of Cell Markers
2
In Situ Proximity Ligation Assay for Connexin Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In situ PLA ( Söderberg et al., 2006 ) (link) has been previously proven to work on cochlear tissues, and especially with membrane proteins ( Defourny et al., 2019a ) . Whole-mount cochleae were treated and handled as for immunolabeling (see above). Anti-connexin 26 (rabbit polyclonal antibody; 1:500; Invitrogen; RRID: AB_2533903) and anti-connexin 30 (mouse monoclonal antibody; 1:100; Santa Cruz Biotechnology; sc-514847) primary antibodies were incubated with tissues overnight at 4 °C. Oligo-labeled anti-mouse plus and anti-rabbit minus probes (Duolink, Olink Biosciences; DUO92101) were then used as recommend by the manufacturer. Negative control was obtained by omitting one of the primary antibody (anticonnexin 30). Tissues were finally labeled for lipid rafts using FITC-conjugated Cholera toxin B subunit (1 μg/mL; Sigma-Aldrich; C1655).
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