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Ortho nitrophenyl β d galactopyranoside onpg

Manufactured by Merck Group
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Sourced in Germany, United States

Ortho-nitrophenyl-β-D-galactopyranoside (ONPG) is a chromogenic substrate used in biochemical assays. It serves as a substrate for the enzyme β-galactosidase, which catalyzes the hydrolysis of ONPG to release the yellow-colored compound ortho-nitrophenol.

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Lab products found in correlation

Porcine skin gelatine, by Merck Group (1 mentions) Phenolphthalein β d glucuronide, by Merck Group (1 mentions) Prebiotic inulin, by Cargill (1 mentions) M nitrobenzoic acid, by Merck Group (1 mentions) Nitrophenyl β d glucoside, by Merck Group (1 mentions)

Close spelling variants of this product

Ortho-nitrophenyl-β-galactopyranoside(ONPG) (2 citations)

2 protocols using ortho nitrophenyl β d galactopyranoside onpg

1

Porcine Skin Gelatine-based Assay

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Porcine skin gelatine, ortho-nitrophenyl-β-D-galactopyranoside (ONPG) and β-galactosidase from Kluyveromyces lactis (EC 3.2.1.23) were purchased from Sigma-Aldrich (Germany). Microbial transglutaminase Activa®WM (mTGase) was kindly donated by Ajinomoto (Japan). Other reagents, all of analytical grade, were supplied by Avantor Performance Materials (Poland).
, & Labus K. (2018). Effective detection of biocatalysts with specified activity by using a hydrogel-based colourimetric assay – β-galactosidase case study. PLoS ONE, 13(10), e0205532.
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2

Analytical-grade Chemical Protocols

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All chemicals and reagents were analytical grade and were purchased form Merck Chemicals (Darmstadt, Germany) unless otherwise stated. The DMH, m-nitrobenzoic acid, phenolphthalein-β-d-glucuronide, nitrophenyl-β-d-glucoside, and ortho-nitrophenyl-β-d-galactopyranoside (oNPG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The prebiotic inulin extracted from chicory roots was purchased from Cargill® (Minneapolis, MN, USA).
Qamar T.R., Syed F., Nasir M., Rehman H., Zahid M.N., Liu R.H, & Iqbal S. (2016). Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats. Nutrients, 8(8), 465.
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