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2 protocols using 1 4 benzoquinone 1 4 bq

1

Redox Substrate Characterization Protocol

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Electron-acceptor substrates from Sigma Aldrich): coenzyme Q1, coenzyme Q10, DCPIP (2,6-dichloroindophenol sodium salt hydrate), potassium ferricyanide, 1,4-benzoquinone (1,4-BQ), ferrocenium hexafluorophosphate (Fc+), diethyl oxalacetate sodium salt, sodium fumarate dibasic, methyl red sodium (crystalline), 3,3′-methylene-bis(4-hydroxycoumarin). Electron acceptor substrates from Carl Roth (Germany): sodium thiosulphate anhydrous, DL-malic acid. Electron-donor substrates from Sigma Aldrich: β-nicotinamide adenine dinucleotide phosphate and reduced tetra (cyclohexylammonium) salt (NADPH).
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2

Modulation of Inflammatory Pathways

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1,4-Benzoquinone (1,4-BQ), Thiazolyl Blue Tetrazolium Bromideand (MTT), and Dimethyloxalylglycine (DMOG, TET2 inhibitor), Ac-YVAD-cmk (Casp1 inhibitor) were purchased from Sigma-Aldrich (USA). FAM-FLICA® Caspase-1 Assay Kit was purchased from ImmunoChemistry Technologies (USA)., Irreversible caspase-1inhibitor (ab141388) was purchased from Abcam (UK). RevertAid First Strand cDNA Synthesis Kit was obtained from ThermoFisher Scientific (USA) while KAPA SYBR® Fast Mastermix were acquired from KAPABiosystems(USA). Human IL1β ELISA Kit, Human IL6 ELISA Kit, Human IL8 ELISA Kit, Human IL10 ELISA Kit, and Goat Anti-Rabbit IgG H&L (FITC), were acquired from Abcam (UK). TET2 antibodies, Aim2, CASP1, IL1β, β-actin and anti-mouse/rabbit IgG antibody were purchased from Cell Signaling Technologie (USA) while GSDMD was acquired from Proeintech.
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