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Pcmv renilla plasmid

Manufactured by Promega
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The PCMV-Renilla plasmid is a vector that contains the Renilla luciferase gene under the control of the CMV promoter. It is used as a reporter for gene expression studies.

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Lab products found in correlation

Dual light luciferase system, by Promega (1 mentions) Pegfp n3, by Takara Bio (1 mentions) Pcmv 3tag 1a, by Agilent Technologies (1 mentions)

2 protocols using pcmv renilla plasmid

1

PATZ1 3'UTR Luciferase Assay

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For Luciferase assays, the pCMV-Renilla plasmid (Promega, Mannheim, Germany) was co-transfected with 3′UTR region of PATZ1 Variant 1/2. Luciferase and Renilla activities were assessed with the Dual-Light Luciferase system (Promega), according to the manufacturer’s protocol, 72 h after the transfection. Luciferase activity was normalized for the Renilla activity.
Vitiello M., Valentino T., De Menna M., Crescenzi E., Francesca P., Rea D., Arra C., Fusco A., De Vita G., Cerchia L, & Fedele M. (2016). PATZ1 is a target of miR-29b that is induced by Ha-Ras oncogene in rat thyroid cells. Scientific Reports, 6, 25268.
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2

Cloning and Characterization of EAF2 and FOXA1

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CMV-Myc and pEGFP-N3 vectors were purchased from Clontech and pCMV-3Tag-1A from Agilent Technologies. Human EAF2 cDNA was amplified by PCR and cloned into the pEGFP-N3, pCMV-Myc and pCMV-3Tag-1A vectors, producing the plasmids of pEGFP-EAF2, Myc-EAF2 and Flag-EAF2. EAF2 deletion mutants were generated previously by PCR and cloned into the pCMV-Myc (23 (link)). pCMV-Renilla plasmid was obtained from Promega and pPSA6.1Luc, a PSA promoter-driven luciferase reporter (24 (link)), was a generous gift from Dr. Marianne Sadar, Ph.D. (BC Cancer Agency, British Columbia, Canada). The PSA promoter is 6.1kb, located from −6kb to +12bp (24 (link)). pCMV6-FOXA1 plasmid was purchased from Origene (RC20604); pEGFP-N3-FOXA1 was generated from pCMV6-FOXA1 by PCR and subcloned into pEGFP-N3 and untagged FOXA1 were also generated from pCMV6-FOXA1 by digestion and ligation.
Guo W., Keener A.L., Jin Y., Cai L., Ai J., Zhang J., Fu G, & Wang Z. (2015). FOXA1 modulates EAF2 regulation of AR transcriptional activity, cell proliferation and migration in prostate cancer cells. The Prostate, 75(9), 976-987.
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