All donors were unaffected by any systemic and oral diseases. The gingival tissues were collected from oral cavity without inflammation. The tissues were then subjected for de-epithelialization and were washed several times with 1× phosphate buffered saline (PBS) (Li StarFish, Milan, Italy). Consequently, the tissues were cultured in serum free, chemically defined medium for the growth of human MSCs (TheraPEAK™ MSCGM-CD™ BulletKit; Lonza, Basel, Switzerland) under standard cell culture conditions. Medium was replaced with fresh medium twice a week. Explants-derived adhered cells were grown until 80%, detached using Triple Select (Li StarFish, Milan, Italy), and subcultured for further experiments. The cytofluorimetric evaluation of stem cell markers has been carried out as previously reported by Libro et al.23 (link)
Therapeak mscgm cd bulletkit
Manufactured by Lonza
Sourced in Switzerland
The TheraPEAK™ MSCGM-CD™ BulletKit is a laboratory equipment product developed by Lonza. It is designed to support the culture and expansion of mesenchymal stem cells (MSCs) in a defined, serum-free medium. The kit provides the necessary components to facilitate the growth and maintenance of MSCs in a controlled and optimized environment.
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Lab products found in correlation
2 protocols using therapeak mscgm cd bulletkit
1
Isolation and Culture of Gingival MSCs
2
Isolation and Expansion of Gingival Mesenchymal Stem Cells
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Cells were isolated from human gingival tissue biopsies from two different donors as previously described [21 (link)]. Biopsies were washed with phosphate-buffered saline (PBS) (Lonza, Basel, Switzerland), cut into small pieces and then placed in a TheraPEAK™MSCGM-CD™ Bullet Kit serum free, chemically defined medium for the growth of human mesenchymal stem cells (MSCGM-CD, Lonza, Basel, Switzerland) at 37 °C in a controlled atmosphere (5% CO2). The medium was refreshed twice a week. After 2 weeks of culture, cells were spontaneously migrated from the tissue explants. After reaching around 80% of confluence, cells were trypsinized using trypsin-EDTA (Lonza, Basel, Switzerland) and subcultured until passage 2 (P2). All the following experiments were conducted in triplicate and were repeated 3 times.
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