Fas antibody

Cervical cancer tissues and paired adjacent normal tissues were processed for immunohistochemistry. All samples were formalin-fixed, paraffin-embedded, and immunostained with the NUP210 antibody (Abcam, Cambridge, MA, USA) and Fas antibody (Abcam). Quantification was conducted by counting positive cells per field. Data are presented as a mean±standard deviation (SD).

An equal amount of protein sample was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA). The blots were then incubated with NUP210 antibody (Abcam), Fas antibody (Abcam), Histone antibody (Abcam), or anti-GAPDH (MultiScience, Hangzhou, China) antibody at 4℃ overnight, followed by incubation with HRP-conjugated goat anti-rabbit or goat anti-mouse antibody (MultiScience) for 1 hour at room temperature. The relative protein levels were detected using peroxide LumiGLO reagent (Cell Signaling Technology, Danvers, MA, USA) and quantified according to the ratio of gray value to the corresponding GAPDH or histone level. The experiments were repeated three times independently.