For monocyte adhesion assays, human PBMCs were directly incubated with the polymer surfaces for 90 min under static conditions. After incubation, samples were gently washed, fixed with 4% formaldehyde for 20 min at room temperature, and stained for anti-human CD14-FITC (1:20; Sigma Aldrich, Darmstadt, Germany) and DAPI. For each condition, 10 pictures with 10× magnification were taken in a predefined order. Immunofluorescence was detected by an Eclipse 90i Advanced Automated Research Microscope System (Nikon GmbH, Düsseldorf, Germany) and monocyte counting was done using Image J (NIH, Stapleton, NY, USA).
Eclipse 90i advanced automated research microscope system
Manufactured by Nikon
Sourced in Germany
The Eclipse 90i Advanced Automated Research Microscope System is a high-performance research microscope designed for a wide range of applications. It features automated functions, including motorized components and precision control, to facilitate advanced microscopy techniques. The system offers versatile optical configurations and accessories to support various imaging methods.
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Lab products found in correlation
2 protocols using eclipse 90i advanced automated research microscope system
1
Monocyte Adhesion Assay Protocol
2
Leukocyte Velocity Quantification
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To assess leukocyte velocity, a suspension of human PBMCs was stained for CellTrace™ Calcein Red-Orange, AM (Life Technologies GmbH, Darmstadt, Germany) and perfused for 5 min with a shear rate of 50 s−1 over polymer films, respectively, using a parallel plate flow chamber (Provitro GmbH, Berlin, Germany). PBMCs were detected by immunofluorescence (Eclipse 90i Advanced Automated Research Microscope System (Nikon GmbH, Düsseldorf, Germany), tracked, and the average rolling velocity was determined using NIS-Elements AR Imaging Software (Nikon GmbH, Düsseldorf, Germany).
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