Jejunal IECs were lysed in a RIPA buffer containing proteinase inhibitors and an OGA inhibitor. Protein extracts were separated on an SDS-PAGE gel, transferred to nitrocellulose membranes, and blotted with the indicated primary antibodies: OGT (ab96718, Abcam, Shanghai, China), O-GlcNAc (ab2739, Abcam), α-Tubulin (11224-1-AP, Proteintech, Wuhan, China), P-STAT6 (Y641) (ab263947, Abcam), and STAT6 (51073-1-AP, Proteintech). After incubation with HRP-conjugated secondary antibodies, the immune complexes were detected using the ECL detection reagents (Beyotime, Shanghai, China).
α tubulin 11224 1 ap
Manufactured by Proteintech
Sourced in China
α-tubulin (11224-1-AP) is a primary antibody that recognizes the α-tubulin protein, a component of the cytoskeleton. This antibody is suitable for various applications, including Western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Cell Signaling Technology (2 mentions)
Hrp conjugated antibodies, by Jackson ImmunoResearch (2 mentions)
Ecl detection reagent, by Beyotime (1 mentions)
Ab2739, by Abcam (1 mentions)
Ab263947, by Abcam (1 mentions)
Ab96718, by Abcam (1 mentions)
O glcnac, by Abcam (1 mentions)
Mg132, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Alexa fluor555 phalloidin a34055, by Thermo Fisher Scientific (1 mentions)
Ubiquitin, by Santa Cruz Biotechnology (1 mentions)
β actin m1210 2, by Huabio (1 mentions)
E cadherin 610182, by BD (1 mentions)
Ab154804, by Abcam (1 mentions)
Ab45688, by Abcam (1 mentions)
Alpha tubulin, by Proteintech (1 mentions)
Anti gapdh sc 365062, by Santa Cruz Biotechnology (1 mentions)
5 protocols using α tubulin 11224 1 ap
1
Protein Expression Analysis of Jejunal IECs
2
Antibodies and Reagents for Cell Signaling Research
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Antibodies against human CD147 was produced by our lab; Par3 (07‐330) and integrin α5 (MAB1956Z) were obtained from Merck Millipore (Darmstadt, Germany); β‐catenin (51067‐2‐AP), occludin (13409‐1‐AP), lamin B (66045‐1‐AP), α‐tubulin (11224‐1‐AP), and Src (60315‐1‐AP) were purchased from Proteintech (Wuhan, China); phosphotyrosine (ARR8004) was obtained from Antibody Revolution (South San Francisco, CA); E‐cadherin (ET1607‐75) and β‐actin (M1210‐2) were purchased from Huabio (Hangzhou, China); multidrug resistance–associated protein 2 (MRP2, ab3373) and mouse CD147 (ab34016) were obtained from Abcam (Cambridge, UK); ZO‐1 (sc‐10804), integrin β1 (sc‐13590), phosphorylated Src (Tyr416, sc‐101802), and ubiquitin (sc‐271289) were purchased from Santa Cruz Biotechnology (Dallas, TX); Na+/taurocholate cotransporting polypeptide (orb13624), mouse MRP2 (orb11075), and CBLL1/Hakai (orb2538) were obtained from Biorbyt (Cambridge, UK); E‐cadherin (610182) was from BD Biosciences (Franklin Lakes, NJ); Alexa Fluor 555 phalloidin (A34055) was from Invitrogen (Carlsbad, CA); CellLight Lysosomes‐RFP (C10597) was purchased from Cell Signaling Technology; Src family activator (sc‐3052) was purchased from Santa Cruz Biotechnology; Src kinase inhibitor Src I‐1, MG132, and chloroquine were from Sigma (St. Louis, MO); TGF‐β1 (100‐21‐10VG) was obtained from Peprotech (Rocky Hill, NJ).
3
Protein Expression Analysis via Western Blot, IF and IHC
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NaCl solution (0.9%) was purchased from Baxter China. Anti-PFKP antibody (GTX35238, 1:100-1:1000), PKM2 (CST, 4053, 1:1,000), HK1(CST, 2024,1:800) and LDHA(Sigma Aldrich, QC52376, 1:1000) were used for Western blotting, immunofluorescence (IF) staining and immunohistochemical (IHC) staining. The primary antibodies for Western blotting against PFKM (ab154804, 1:1000), and PFKL (ab45688, 1:1000) were all from Abcam (Cambridge, MA). The primary antibodies for Western blotting against ROCK1 (21850-1-AP, 1:1000), RhoA (10749-1-AP, 1:1000), α-tubulin (11224-1-AP, 1:1000), ALDOB (18065-1-AP,1:1000), and alpha-tubulin(11224-1-AP,1:1000) were purchased from proteintech (Wuhan, China). Anti-GAPDH (sc-365062, 1:1000) was purchased from Santa Cruz (Santa Cruz, CA). DAPI and secondary antibodies against rabbit IgG-HRP(ANT020), IgG-HRP (ANT019), Alexa Fluor 488, 594 conjugated anti-mouse IgG, and anti-rabbit IgG were obtained from Antgene (Wuhan, China).
4
Quantifying Protein Expression in Breast Cancer
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Western blot Total protein was extracted from breast cancer cells and tumor tissues in RIPA lysis buffer and separated in 8% or 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before electrotransferred onto polyvinylidene di uoride (PVDF) membrane as previously described. The membrane was incubated with primary antibody overnight at 4°C. Subsequently, the membrane was incubated with secondary antibody for 1 h at room temperature. Immunoreactive bands on the membrane were visualized by the enhanced chemiluminescence assay. The primary antibodies to Par3 (11085-1-AP, 1:1000 dilution, Proteintech, China), α-tubulin (11224-1-AP, 1:5000 dilution, Proteintech, China) and β-actin (#3700, 1:3000 dilution, Cell signaling) were commercially obtained. The secondary monoclonal or polyclonal HRP-conjugated antibodies (1:10000) were purchased from Jackson Immunoresearch (USA).
5
Quantifying Protein Expression in Breast Cancer
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Western blot Total protein was extracted from breast cancer cells and tumor tissues in RIPA lysis buffer and separated in 8% or 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before electrotransferred onto polyvinylidene di uoride (PVDF) membrane as previously described. The membrane was incubated with primary antibody overnight at 4°C. Subsequently, the membrane was incubated with secondary antibody for 1 h at room temperature. Immunoreactive bands on the membrane were visualized by the enhanced chemiluminescence assay. The primary antibodies to Par3 (11085-1-AP, 1:1000 dilution, Proteintech, China), α-tubulin (11224-1-AP, 1:5000 dilution, Proteintech, China) and β-actin (#3700, 1:3000 dilution, Cell signaling) were commercially obtained. The secondary monoclonal or polyclonal HRP-conjugated antibodies (1:10000) were purchased from Jackson Immunoresearch (USA).
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