The fixed tissues were dehydrated and fixed with paraffin in a 2T-12M tissue processor (Xiaogan Yaguang Medical Electronic Technology Corporation Ltd., Xiaogan, PR China) and then embedded in paraffin blocks using an embedding system (Leica, Germany, UK). The blocks were then sliced into 7 μm thick sections using a rotary microtome (Leica, Germany, UK) and stained with H&E. The crypt depth (Cd) (from the basis of the villus to the submucosa), the villus height (Vh) (from the tip of the villus to the crypt), and the Vh/Cd ratio were evaluated (11 (link)). Histomorphology analyses were performed on 6 well-oriented and intact villi and 6 crypts chosen from the duodenum, jejunum, ileum, and cecum. Slices were photographed and measured using an EVOS™ M5000 Imaging System (Thermo Fisher Scientific, Waltham, Massachusetts, USA).
Embedding system
Manufactured by Leica
Sourced in Germany
The Embedding system is a core piece of laboratory equipment designed for the preparation of tissue samples for microscopic analysis. It allows for the embedding of tissue specimens in a supportive medium, such as paraffin, to facilitate thin sectioning and mounting on slides for further examination.
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Lab products found in correlation
3 protocols using embedding system
1
Histomorphometric Analysis of Intestinal Tissues
2
Histological Analysis of Testicular Tissues
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Testicular tissues were collected and submerged in buffered formalin (10% and pH 7.4) for 24 h before dehydrating and fixing with paraffin in a 2T-12M tissue processor (XiaoganYaguang Medical Electronic, Xiaogan, China). The fixed tissues were embedded in paraffin blocks using an embedding system (Leica, Germany). The sections were then sliced into a diameter of 5 μm using a rotary microtome (Leica, Germany) and further processed for hematoxylin-eosin (HE) staining. The seminiferous tubule diameter (STD) and seminiferous tubule epithelial height (SEH) were measured using an EVOS™ M5000 Imaging System (ThermoFisher Scientific, MA, USA). STD was defined as the shortest distance between the outer edge of the tubule and the SEH between the germ cells.
3
Histomorphology Analysis of Intestinal Tissue
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The xed tissues were dehydrated and xed with para n in a 2T-12M tissue processor (XiaoganYaguang Medical Electronic Technology Co., Ltd., Xiaogan, P. R. China), and then embedded in para n blocks using an embedding system (Leica, Germany). The blocks were then sliced into 7 µm-thick sections using a rotary microtome (Leica, Germany) and stained with Haematoxylin & Eosin (HE). The crypt depth (Cd, from the basis of the villus to the submucosa), villus height (Vh, from the tip of the villus to the crypt) and the villus height to crypt depth (Vh/Cd) ratio were evaluated [22] . Histomorphology analyses were performed on 6 well-oriented and intact villi and 6 crypts chosen from duodenum, jejunum, ileum and cecum. Slices were photographed and measured using an EVOS™ M5000 Imaging System (Thermo Fisher Scienti c, America).
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