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Lysm cre b6.129p2 lyz2tm1 cre lfo j

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LysM-Cre (B6.129p2-Lyz2tm1(cre)lfo/J) is a genetically engineered mouse strain that expresses Cre recombinase under the control of the lysozyme M (LysM) gene promoter. This allows for Cre-mediated gene manipulation specifically in myeloid cells, including macrophages and neutrophils.

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C57bl 6j, by Jackson ImmunoResearch (2 mentions)

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LysM-Cre (106 citations) B6.129P2-Lyz2tm1(cre) (2 citations) B6.129P2-Lyz2tm1(cre)lfo/J (LysM-Cre) mice (2 citations)

2 protocols using lysm cre b6.129p2 lyz2tm1 cre lfo j

1

Generation and Use of Genetically Modified Mice

Cited 1 time
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C57BL/6/J and LysM-Cre (B6.129p2-Lyz2tm1(cre)lfo/J) mice were purchased from Jackson Laboratory and housed in the animal facility of University of Lausanne. Macrophage-specific Cas9 knock-in were generated as previously described29 (link). Macrophage-specific XBP1 Knockout mice were generated by crossing LysM-Cre mice (myeloid-specific overexpression of Cre recombinase) with XBP1fl/fl mice. Macrophages-specific Lpcat3 knockout mice were generated by crossing LysM-Cre mice with Lpcat3fl/fl mice53 (link). BRafCA; Tyr::CreER; Ptenlox4-5 (Braf/PTEN) was obtained from M. Bonsenburg at Yale University. Bone marrow of Mincle-knockout mice was provided by David Sancho54 (link). All experiments were performed in accordance with Swiss federal regulations and procedures ethically approved by veterinary authority of Canton Vaud.
Di Conza G., Tsai C.H., Gallart-Ayala H., Yu Y.R., Franco F., Zaffalon L., Xie X., Li X., Xiao Z., Raines L.N., Falquet M., Jalil A., Locasale J.W., Percipalle P., Masson D., Huang S.C., Martinon F., Ivanisevic J, & Ho P.C. (2021). Tumor-induced reshuffling of lipid composition on the ER membrane sustains macrophage survival and pro-tumorigenic activity. Nature immunology, 22(11), 1403-1415.
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2

Generation and Use of Genetically Modified Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6/J and LysM-Cre (B6.129p2-Lyz2tm1(cre)lfo/J) mice were purchased from Jackson Laboratory and housed in the animal facility of University of Lausanne. Macrophage-specific Cas9 knock-in were generated as previously described29 (link). Macrophage-specific XBP1 Knockout mice were generated by crossing LysM-Cre mice (myeloid-specific overexpression of Cre recombinase) with XBP1fl/fl mice. Macrophages-specific Lpcat3 knockout mice were generated by crossing LysM-Cre mice with Lpcat3fl/fl mice53 (link). BRafCA; Tyr::CreER; Ptenlox4-5 (Braf/PTEN) was obtained from M. Bonsenburg at Yale University. Bone marrow of Mincle-knockout mice was provided by David Sancho54 (link). All experiments were performed in accordance with Swiss federal regulations and procedures ethically approved by veterinary authority of Canton Vaud.
Di Conza G., Tsai C.H., Gallart-Ayala H., Yu Y.R., Franco F., Zaffalon L., Xie X., Li X., Xiao Z., Raines L.N., Falquet M., Jalil A., Locasale J.W., Percipalle P., Masson D., Huang S.C., Martinon F., Ivanisevic J, & Ho P.C. (2021). Tumor-induced reshuffling of lipid composition on the ER membrane sustains macrophage survival and pro-tumorigenic activity. Nature immunology, 22(11), 1403-1415.
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