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Mouse anti flag tag

Manufactured by Cell Signaling Technology
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The Mouse anti-Flag tag is a monoclonal antibody that recognizes the Flag epitope tag. The Flag tag is a peptide sequence that can be fused to a protein of interest, allowing for detection and purification of the tagged protein.

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Lab products found in correlation

Bafa1, by Merck Group (2 mentions) Anti flag tag, by Cell Signaling Technology (2 mentions) Rabbit anti gapdh antibody, by GeneTex (2 mentions) Goat anti ace2 antibody, by R&D Systems (2 mentions) Rabbit anti adam9, by Cell Signaling Technology (2 mentions) Mouse anti hiv p24 antibody, by Abcam (2 mentions) Rabbit anti ha tag antibody, by Merck Group (2 mentions) Normal rabbit igg, by Santa Cruz Biotechnology (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Poly l lysine solution, by Merck Group (2 mentions) Nh4cl, by Merck Group (2 mentions) Mouse anti β actin, by BD (1 mentions)

3 protocols using mouse anti flag tag

1

Antibody Procurement for Protein Detection

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Rabbit anti-ADAM9, anti-Flag tag, and mouse anti-Flag tag antibodies were purchased from Cell Signaling Technology (2099s, 2368s, and 8146s). Mouse anti-HIV p24 antibody was purchased from Abcam (ab9071). Rabbit anti-GAPDH antibody was purchased from GeneTex (GTX100118). Rabbit anti-HA tag antibody was purchased from Millipore (04–902). Normal rabbit IgG was purchased from Santa Cruz (sc-2027). Goat anti-ACE2 antibody was purchased from R&D Systems (AF933). All Alexa Fluor-conjugated secondary antibodies used for immunofluorescence were procured from Molecular Probes (Invitrogen). NH4Cl, BafA1, and poly-L-lysine solution (P4707) were purchased from Sigma-Aldrich. Batimastat (BB-94) was purchased from Calbiochem (196440).
Melano I., Cheng W.C., Kuo L.L., Liu Y.M., Chou Y.C., Hung M.C., Lai M.M., Sher Y.P, & Su W.C. (2023). A disintegrin and metalloproteinase domain 9 facilitates SARS-CoV-2 entry into cells with low ACE2 expression. Microbiology Spectrum, 11(5), e03854-22.
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2

Antibody Procurement for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit anti-ADAM9, anti-Flag tag, and mouse anti-Flag tag antibodies were purchased from Cell Signaling Technology (2099s, 2368s, and 8146s). Mouse anti-HIV p24 antibody was purchased from Abcam (ab9071). Rabbit anti-GAPDH antibody was purchased from GeneTex (GTX100118). Rabbit anti-HA tag antibody was purchased from Millipore (04–902). Normal rabbit IgG was purchased from Santa Cruz (sc-2027). Goat anti-ACE2 antibody was purchased from R&D Systems (AF933). All Alexa Fluor-conjugated secondary antibodies used for immunofluorescence were procured from Molecular Probes (Invitrogen). NH4Cl, BafA1, and poly-L-lysine solution (P4707) were purchased from Sigma-Aldrich. Batimastat (BB-94) was purchased from Calbiochem (196440).
Melano I., Cheng W.C., Kuo L.L., Liu Y.M., Chou Y.C., Hung M.C., Lai M.M., Sher Y.P, & Su W.C. (2023). A disintegrin and metalloproteinase domain 9 facilitates SARS-CoV-2 entry into cells with low ACE2 expression. Microbiology Spectrum, 11(5), e03854-22.
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3

Immunoblot Analysis of Scl-CreER and Scl:Cdx2 Cells

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Immunoblot for Scl-CreERT and Scl:Cdx2 cells was performed using mouse anti-CDX2 monoclonal antibody (CDX-88; Abcam) (1:1000) and mouse anti-β-actin (BD Biosciences) (1:5000). Immunoblot for rabbit anti-Flag immunoprecipitation of Ba/F3 cells transduced with pMIG-Cdx2-Flag was performed using mouse anti-Flag tag monoclonal antibody (8146, Cell Signaling) (1:1000).
Vu T., Straube J., Porter A.H., Bywater M., Song A., Ling V., Cooper L., Pali G., Bruedigam C., Jacquelin S., Green J., Magor G., Perkins A., Chalk A.M., Walkley C.R., Heidel F.H., Mukhopadhyay P., Cloonan N., Gröschel S., Mallm J.P., Fröhling S., Scholl C, & Lane S.W. (2020). Hematopoietic stem and progenitor cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia. Nature Communications, 11, 3021.
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