Apoptosis and necrosis were analyzed by double staining with annexin V-FITC and 7-AAD. Annexin V binds to the cells with exposed phosphatidylserine, whereas 7-AAD labels the cells with membrane damage. Apoptotic cells were detected using the Annexin V-FITC/7-AAD Kit (Apoptosis Detection Kit, Beckman Coulter, USA). Staining was performed according to the manufacturer’s instructions and Shounan protocol (Shounan et al., 1998 (link)). After the treatment with compounds 0 , 3 , and 4 (Fig. 1 . ) at a concentration of 1 μM and 10 μM, the cells were collected, washed in PBS and resuspended in ice cold binding buffer. Ten thousand events were analyzed on FC500 Beckman Coulter Flow Cytometer (Nyon, Switzerland). The percent of viable (Annexin V−7-AAD-) cells, early apoptotic (Annexin V+7-AAD-) cells, late apoptotic (Annexin V+7-AAD+) cells, and necrotic cells (Annexin V−7-AAD+) cells were evaluated by Flowing Software (http://www.flowingsoftware.com/ ).
Annexin 5 fitc 7 aad kit apoptosis detection kit
Manufactured by Beckman Coulter
Sourced in United States
The Annexin V-FITC/7-AAD Kit is a laboratory reagent used for the detection and analysis of apoptosis. The kit contains Annexin V-FITC, a fluorescent protein that binds to phosphatidylserine, and 7-AAD, a DNA-binding dye. These components allow for the identification of cells undergoing early and late stages of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 fitc 7 aad kit apoptosis detection kit
1
Apoptosis and Necrosis Analysis
2
Annexin V-FITC/7-AAD Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis and necrosis were analyzed by double staining with Annexin V-FITC and 7-AAD. Annexin V binds to the cells with exposed phosphatidylserine, whereas 7-AAD labels the cells with membrane damage. Apoptotic cells were detected using the Annexin V-FITC/7-AAD Kit (Apoptosis Detection Kit, Beckman Coulter, Brea, CA, USA). Staining was performed according to the manufacturer’s instructions and Shounan protocol [46 (link)]. After the treatment with OG1 and OG2 at a concentration of 10 µg/mL and 100 µg/mL, the cells were collected, washed in PBS, and resuspended in ice-cold binding buffer. Ten thousand events were analyzed on Flow Cytometer Cytomics FC500 (Beckman Coulter, USA). The percent of viable (Annexin V−7-AAD−) cells, early apoptotic (Annexin V+7-AAD−) cells, late apoptotic (Annexin V+7-AAD+) cells, and necrotic cells (Annexin V−7-AAD+) cells were evaluated by Flowing Software (http://www.flowingsoftware.com/ , accessed on 27 January 2021).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!