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96 well tissue culture microplates

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The 96-well tissue culture microplates are a versatile laboratory equipment designed for a variety of cell-based assays and experiments. These microplates provide a standardized format for culturing and analyzing cells in a high-throughput manner.

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Lab products found in correlation

Wst 1 cell proliferation assay kit, by Cayman Chemical (1 mentions)

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96-well microplates (290 citations) 96 wells (10 citations) 96-well culture microplates (3 citations) 96-well flat clear bottom polystyrene tissue-culture treated microplates (2 citations)

2 protocols using 96 well tissue culture microplates

1

Cytotoxicity Evaluation of WPE Extract

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The cytotoxicity of WPE was determined by measuring the effect on cell morphology after 24, 48, and 72 h, by light microscopy and cell viability evaluation through the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay in 96-well tissue culture microplates (Corning). The CD50 was defined as the concentration of the extract that inhibited 50% cell growth when compared with the control cultures [77 (link)].
D’Angeli F., Malfa G.A., Garozzo A., Li Volti G., Genovese C., Stivala A., Nicolosi D., Attanasio F., Bellia F., Ronsisvalle S, & Acquaviva R. (2021). Antimicrobial, Antioxidant, and Cytotoxic Activities of Juglans regia L. Pellicle Extract. Antibiotics, 10(2), 159.
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2

WST-1 Assay for Cell Proliferation

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Cell growth was measured using the WST-1 cell proliferation assay kit (Cayman Chemical, Ann Arbor, MI, USA). Briefly, cells were seeded as in the cytotoxicity assay in 100 μL per well of 96-well tissue culture microplates (Corning Incorporated-Life Sciences). Quiescent cells were treated with PFD (0.0-1.0 mg/mL) either 24 h prior to, concurrent with, or 24 h following TGF-β1 stimulation. NHDF were pulsed with 10 μL/well WST-1 reagent and incubated at 37 °C for two hours. Absorbance at 450 nm was measured over the course of four days as an indicator of proliferation.
Hall C.L., Wells A.R, & Leung K.P. (2018). Pirfenidone reduces profibrotic responses in human dermal myofibroblasts, in vitro. Laboratory investigation; a journal of technical methods and pathology, 98(5).
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