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Massarray typer 4.0 analyzer software

Manufactured by Agena
Sourced in United States

The MassARRAY Typer 4.0 Analyzer software is a tool designed for analyzing genetic data. It provides a platform for processing and interpreting results from mass spectrometry-based genetic assays.

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4 protocols using massarray typer 4.0 analyzer software

1

Genotyping of BMP15 and GDF9 Variants

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The FecXR, FecXGr, FecXO, and FecXBar in BMP15, and the FecGT, FecGV, FecGA, and FecG1 in GDF9 genes, were genotyped with the MassARRAY® SNP genotyping system (Agena Bioscience, San Diego, CA, USA) in the 260 MG population. PCR and extension primers were designed from sequences containing each target mutation and ~100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software) using the default settings. The genotype of each allele was analyzed using the Sequenom MassARRAY iPLEX platform [37 (link)]. The resulting data were analyzed using the MassARRAY Typer 4.0 Analyzer software (Agena Bioscience, San Diego, CA, USA).
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2

Genotyping Qinchuan Cattle SNPs

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For the population consisting of 350 Qinchuan cattle, the four target SNPs were genotyped with the MassARRAY®® SNP genotyping system (Agena Bioscience, San Diego, CA, USA). PCR and extension primers were designed from sequences containing each target mutation and ~100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software), using the default settings. The genotype of each SNP was analyzed using the Sequenom MassARRAY iPLEX platform (Sequenom, San Diego, CA, USA) [15 (link)]. The resulting data were analyzed using the MassARRAY Typer 4.0 Analyzer software (Agena Bioscience, San Diego, CA, USA).
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3

SNP Genotyping of Sonid Sheep Variants

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Four target variants were genotyped with the MassARRAY® SNP genotyping system (Agena Bioscience, San Diego, CA, USA) in the 378 Sonid sheep. PCR and extension primers were designed from sequences containing each target mutation and ~100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software) using the default settings (Table 2). The genotype of each SNP was analyzed using the Sequenom MassARRAY iPLEX platform (Sequenom, San Diego, CA, USA) [19 (link)]. The resulting data were analyzed using MassARRAY Typer 4.0 Analyzer software (Agena Bioscience, San Diego, CA, USA).
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4

Genotyping of Cattle Genetic Variants

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For 350 QC cattle population, the c.*188G>A SNP in AKIRIN2, the g.231054C>T SNP in TTN, the g.1471620G>T SNP in EDG1, and the g.70014208A>G SNP in the MYBPC1 genes were genotyped with the MassARRAY ® SNP genotyping system (Agena Bioscience, San Diego, CA, USA). PCR and extension primers were designed from sequences containing each target mutation and ~ 100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software) using the default settings. The genotype of each allele was analyzed using the Sequenom MassARRAY iPLEX platform [26] . The resulting data was analyzed using the MassARRAY Typer 4.0 Analyzer software (Agena Bioscience).
For 41 MGC, 50 MGM, 50 WL, 50 LL and 24 LX cattle populations, the c.*188G>A SNP in AKIRIN2, the g.231054C>T SNP in TTN, the g.1471620G>T SNP in EDG1, and the g.70014208A>G SNP in the MYBPC1 genes were genotyped using PCR-restriction fragment length polymorphism (RFLP) method as described previously [13, 14, 16, 17] . The PCR primers and restriction enzymes used for PCR-RFLP are shown in Table S1.
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