The FecXR, FecXGr, FecXO, and FecXBar in BMP15, and the FecGT, FecGV, FecGA, and FecG1 in GDF9 genes, were genotyped with the MassARRAY® SNP genotyping system (Agena Bioscience, San Diego, CA, USA) in the 260 MG population. PCR and extension primers were designed from sequences containing each target mutation and ~100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software ) using the default settings. The genotype of each allele was analyzed using the Sequenom MassARRAY iPLEX platform [37 (link)]. The resulting data were analyzed using the MassARRAY Typer 4.0 Analyzer software (Agena Bioscience, San Diego, CA, USA).
Massarray typer 4.0 analyzer software
Manufactured by Agena
Sourced in United States
The MassARRAY Typer 4.0 Analyzer software is a tool designed for analyzing genetic data. It provides a platform for processing and interpreting results from mass spectrometry-based genetic assays.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using massarray typer 4.0 analyzer software
1
Genotyping of BMP15 and GDF9 Variants
2
Genotyping Qinchuan Cattle SNPs
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For the population consisting of 350 Qinchuan cattle, the four target SNPs were genotyped with the MassARRAY®® SNP genotyping system (Agena Bioscience, San Diego, CA, USA). PCR and extension primers were designed from sequences containing each target mutation and ~100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software ), using the default settings. The genotype of each SNP was analyzed using the Sequenom MassARRAY iPLEX platform (Sequenom, San Diego, CA, USA) [15 (link)]. The resulting data were analyzed using the MassARRAY Typer 4.0 Analyzer software (Agena Bioscience, San Diego, CA, USA).
3
SNP Genotyping of Sonid Sheep Variants
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Four target variants were genotyped with the MassARRAY® SNP genotyping system (Agena Bioscience, San Diego, CA, USA) in the 378 Sonid sheep. PCR and extension primers were designed from sequences containing each target mutation and ~100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software ) using the default settings (Table 2 ). The genotype of each SNP was analyzed using the Sequenom MassARRAY iPLEX platform (Sequenom, San Diego, CA, USA) [19 (link)]. The resulting data were analyzed using MassARRAY Typer 4.0 Analyzer software (Agena Bioscience, San Diego, CA, USA).
4
Genotyping of Cattle Genetic Variants
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For 350 QC cattle population, the c.*188G>A SNP in AKIRIN2, the g.231054C>T SNP in TTN, the g.1471620G>T SNP in EDG1, and the g.70014208A>G SNP in the MYBPC1 genes were genotyped with the MassARRAY ® SNP genotyping system (Agena Bioscience, San Diego, CA, USA). PCR and extension primers were designed from sequences containing each target mutation and ~ 100 upstream and downstream bases with Assay Design Suite (http://agenabio.com/assay-design-suite-20-software) using the default settings. The genotype of each allele was analyzed using the Sequenom MassARRAY iPLEX platform [26] . The resulting data was analyzed using the MassARRAY Typer 4.0 Analyzer software (Agena Bioscience).
For 41 MGC, 50 MGM, 50 WL, 50 LL and 24 LX cattle populations, the c.*188G>A SNP in AKIRIN2, the g.231054C>T SNP in TTN, the g.1471620G>T SNP in EDG1, and the g.70014208A>G SNP in the MYBPC1 genes were genotyped using PCR-restriction fragment length polymorphism (RFLP) method as described previously [13, 14, 16, 17] . The PCR primers and restriction enzymes used for PCR-RFLP are shown in Table S1.
For 41 MGC, 50 MGM, 50 WL, 50 LL and 24 LX cattle populations, the c.*188G>A SNP in AKIRIN2, the g.231054C>T SNP in TTN, the g.1471620G>T SNP in EDG1, and the g.70014208A>G SNP in the MYBPC1 genes were genotyped using PCR-restriction fragment length polymorphism (RFLP) method as described previously [13, 14, 16, 17] . The PCR primers and restriction enzymes used for PCR-RFLP are shown in Table S1.
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