Cytochalasin d cyt d

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Cytochalasin D (Cyt D) is a fungal metabolite that acts as an actin-depolymerizing agent. It binds to the barbed end of actin filaments, inhibiting actin polymerization and disrupting the cytoskeleton.

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Lab products found in correlation

Cellrox deep red, by Thermo Fisher Scientific (1 mentions) Chlorpromazine cpz, by Merck Group (1 mentions) 7 amino actinomycin d, by Cytek Biosciences (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Brefeldin a bfa, by Merck Group (1 mentions) Z vad fmk, by Promega (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Calpeptin, by Bio-Techne (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti β catenin, by Abcam (1 mentions) Polyethylenimine (pei), by Polysciences (1 mentions)

2 protocols using cytochalasin d cyt d

Insect Cell-Based Assay Protocol

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Sf9 insect Cells (Novagen,
St. Louis, MO, USA), sixth instar S. frugiperda (Benzon
Research, Carlisle, PA, USA), 2,2,2-trifluoroethanol
(TFE) (Thermo Fisher, USA), 6-well treated tissue culture plates (Corning
Inc. Corning, NY, USA), 5(6)-carboxytetramethylrhodamine N-succinimidyl ester (Sigma-Aldrich, St. Louis, MO, USA), Grace’s
Insect Medium 1× supplemented (Thermo Fisher, USA), fetal bovine
serum (FBS) (CPS Serum, Parkville, MO, USA), chlorpromazine (CPZ)
(Sigma-Aldrich, St. Louis, MO, USA), dynasore and nystatin (Sigma-Aldrich,
St. Louis, MO, USA), methyl-β-cyclodextrin (M-β-CD) (Millipore
Sigma, USA), cytochalasin D (Cyt D) (Tocris Biosciences, MN, USA),
brefeldin A (BFA) (Sigma-Aldrich, St. Louis, MO, USA), 7-aminoactinomycin
D (7-AAD) (Tonbo, San Diego, CA), paraformaldehyde (Sigma-Aldrich,
St. Louis, MO, USA), CYP450 dsRNA (RNA Greentech, USA), Griess reagent
kit for nitrite quantification (Invitrogen, USA), CellROX Deep Red
(Invitrogen, USA), and Cell Navigatior Lysosome Staining Kit—Green
Fluorescence (AAT Bioquest, Sunnyvale, CA).

McGraw E., Roberts J.D., Kunte N., Westerfield M., Streety X., Held D, & Avila L.A. (2022). Insight into Cellular Uptake and Transcytosis of Peptide Nanoparticles in Spodoptera frugiperda Cells and Isolated Midgut. ACS Omega, 7(13), 10933-10943.

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Cloning and Tagging of Human FAPP1 Protein

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Human FAPP1 cDNA was PCR amplified from the HeLa cell total mRNA and cloned into pcDNA-6.2/N-EmGFP-DEST (for N-terminal tagging) by using Gateway (Invitrogen) cloning technology as described previously (58 (link)). Ii-Str_SBP-EGFP-E-cadherin and Str-KDEL_ManII-SBP-EGFP were purchased from Addgene (plasmids 65288 and 65252). Polyethylenimine (Polysciences) and Lipofectamine 3000 (Invitrogen) were used for transfection. Z-VAD-FMK was purchased from Promega and was used at 50 μM. Cytochalasin D (cytD) was purchased from Tocris Bioscience and used at 5 μg/ml. Z-VAD-FMK or cytD was added 1 h before infection. Calpeptin was purchased from Tocris Bioscience and was used at 20 or 50 μM. Antibodies used for Western blot analysis were anti-E-cadherin (1:1,000) (24E10, 3195; Cell Signaling Technology), anti-β-actin (1:1,000) (13E5, 4970; Cell Signaling Technology), anti-β-catenin (1:100, ab16051; Abcam), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1,000, H12, sc-166574).

Nozawa T., Iibushi J., Toh H., Minowa-Nozawa A., Murase K., Aikawa C, & Nakagawa I. (2021). Intracellular Group A Streptococcus Induces Golgi Fragmentation To Impair Host Defenses through Streptolysin O and NAD-Glycohydrolase. mBio, 12(1), e01974-20.

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