Aa240 model aas atomic absorption spectrometer

Total arsenic in liver was quantified by digestion, using tri-acid mixture of nitric acid, perchloric acid and sulphuric acid (10:4:1) following the method.[12 (link)] The digested samples were diluted with deionized Millipore water, passed through Whatman filter paper No. 4 (Rankem, India) and made the volume to 10ml. Concentrated hydrochloric acid (5 ml) was added to it and shaken well. Then after 1ml of potassium iodide (5% w/v) and ascorbic acid (5% w/v) mixture was added and the aliquot was incubated for 45 min for transformation of arsenate to arsenite. The final volume was made up to 50ml with Millipore water and arsenic concentration read on Varian AA240 model AAS Atomic Absorption Spectrometer (AAS) equipped with vapour generation accessories. The operating parameters were: lamp, arsenic hollow cathode lamp; wavelength, 193.7 nm; slit width, 0.5 nm; lamp current, 10.0 mA; vapor type, air/acetylene; air flow, 10.00 L/min; inert gas for hydride generation, Argon. Reducing agent (Aqueous solution of 0.6% sodium borohydride was prepared in 0.5% w/v sodium hydroxide) and 40% hydrochloric acid (HCl) were prepared freshly before use. The working standards were 5, 10, 20 and 40μg/L and prepared by same procedure as test sample.

Total arsenic in kidney was quantified by digestion, using a tri-acid mixture of nitric acid, perchloric acid and sulphuric acid (10:4:1) following the method of Datta et al.[19 (link)] The digested samples were diluted with deionized Millipore water, passed through Whatman filter paper No. 4 (Rankem, India) and made the volume to 10 ml. Concentrated hydrochloric acid (5 ml) was added to it and shaken well. Then after 1 ml of potassium iodide (5% w/v) and ascorbic acid (5% w/v) mixture was added and the aliquot was incubated for 45 min for transformation of arsenate to arsenite.[20 ] The final volume was made up to 50 ml with Millipore water and arsenic concentration read on the Varian AA240 model AAS Atomic Absorption Spectrometer (AAS) equipped with vapour generation accessories. The operating parameters were: lamp, arsenic hollow cathode lamp; wavelength, 193.7 nm; slit width, 0.5 nm; lamp current, 10.0 mA; vapor type, air/acetylene; air flow, 10.00 L/min; inert gas for hydride generation, Argon. Reducing agent (Aqueous solution of 0.6% sodium borohydride was prepared in 0.5% w/v sodium hydroxide) and 40% HCl were freshly prepared before use. The working standards were 5, 10, 20 and 40 μg/L and prepared by the same procedure as the test sample.