Site-directed mutagenesis was performed using a site-directed mutagenesis kit (Agilent) according to the manufacturer’s guidelines. Mouse Zfp24 coding sequence was cloned into pCMV-AC-GFP mailman expression vector (Origene). For phosphomimetic substitution, serine 274 in the first linker, threonine 302 in the second linker, and threonine 330 in the third linker were replaced by glutamate. For the mutant that mimics the non-phosphorylated state, serine 274, threonine 302, and threonine 330 were replaced by alanine.
Pcmv ac gfp mailman expression vector
Manufactured by OriGene
The PCMV-AC-GFP mailman expression vector is a plasmid designed for the expression of proteins in mammalian cells. It contains a cytomegalovirus (CMV) promoter to drive high-level expression of the gene of interest, and the green fluorescent protein (GFP) gene as a reporter. The vector is suitable for transient or stable transfection experiments in a variety of cell lines.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pcmv ac gfp mailman expression vector
1
Phosphomimetic Zfp24 Mutagenesis
2
Phosphomimetic Zfp24 Mutagenesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Site-directed mutagenesis was performed using a site-directed mutagenesis kit (Agilent) according to the manufacturer’s guidelines. Mouse Zfp24 coding sequence was cloned into pCMV-AC-GFP mailman expression vector (Origene). For phosphomimetic substitution, serine 274 in the first linker, threonine 302 in the second linker, and threonine 330 in the third linker were replaced by glutamate. For the mutant that mimics the non-phosphorylated state, serine 274, threonine 302, and threonine 330 were replaced by alanine.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!