Ab7900ht cycler

Manufactured by Thermo Fisher Scientific

The AB7900HT cycler is a real-time PCR instrument designed for high-throughput gene expression analysis. It features 96-well sample capacity, temperature range from 4°C to 100°C, and supports a variety of sample types and detection chemistries.

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Lab products found in correlation

Magmax 96 total rna isolation kit, by Thermo Fisher Scientific (1 mentions) Specific taqman probes, by Thermo Fisher Scientific (1 mentions) Cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Qiazol lysis reagent, by Qiagen (1 mentions)

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AB7900HT

2 protocols using ab7900ht cycler

Transcriptomic Response to Adrenergic Stimuli

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RNASeq experiments were done comparing untreated cells with a treatment with isoproterenol, adrenaline or noradrenaline for 4 h in THP1 cells.
qPCR was run in THP1 cells for 4 h incubation time with isoproterenol and formoterol at 1, 10 and 100 nM. Total RNAs were isolated with MagMAX™−96 Total RNA Isolation Kit (Ambion ref#AM1830), and cDNA was made using a cDNA Synthesis Kit (Applied Biosystems™ Ref#4368813) RT-PCRs were performed in 384-well plates on an AB7900HT cycler (Applied Biosystems) using specific TaqMan probes (Applied Biosystems). Housekeeper normalization was done relative to the one of the three genes GAPDH, PPIB or TBP, which had the most similar expression level to the gene of interest, according to our DMSO qPCR data. All measurements were done in quadruplicates.

Renner S., Bergsdorf C., Bouhelal R., Koziczak-Holbro M., Amati A.M., Techer-Etienne V., Flotte L., Reymann N., Kapur K., Hoersch S., Oakeley E.J., Schuffenhauer A., Gubler H., Lounkine E, & Farmer P. (2020). Gene-signature-derived IC50s/EC50s reflect the potency of causative upstream targets and downstream phenotypes. Scientific Reports, 10, 9670.

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Hippocampal RNA Extraction and qPCR

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One longitudinally sectioned half of the right hippocampus obtained from the animals belonging to Group B shown in Supplementary Fig. S1 B were used to isolate total RNA with the QIAzol Lysis Reagent (Qiagen) and RNeasy Mini kit (Qiagen). Reverse transcription (RT) of 20 ng/ul RNA was performed using the High capacity cDNA Reverse Transcription kit (Applied Biosystems). qPCR was developed on a AB 7900HT cycler (Applied Biosystems) (384-multi-well plates). 5 ng of cDNA per well was run in triplicate. Data were obtained using the Pfaffl method. The commercial Taqman probes (Applied Biosystems) used are detailed in Supplementary Fig. S4 A.

Llorens-Martín M., Jurado-Arjona J., Bolós M., Pallas-Bazarra N, & Ávila J. (2016). Forced swimming sabotages the morphological and synaptic maturation of newborn granule neurons and triggers a unique pro-inflammatory milieu in the hippocampus. Brain, behavior, and immunity, 53.

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