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S100 protein

Manufactured by Roche
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The S100 protein is a lab equipment product that functions as a calcium-binding protein. It is involved in the regulation of a variety of cellular processes, including cell growth, differentiation, and metabolism. The S100 protein is commonly used in research and clinical settings to study cellular signaling pathways and protein interactions.

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Lab products found in correlation

Benchmark xt, by Roche (4 mentions) Cytokeratin ae1 3, by Merck Group (4 mentions) Desmin, by Agilent Technologies (3 mentions) Calretinin, by Biocare Medical (2 mentions) Ini 1, by BD (2 mentions) Ttf 1 clone 8g7g3 1, by Agilent Technologies (1 mentions) Gata3 clone l50 823, by Cell Marque (1 mentions) Mammaglobin clone 304 1a5, by Agilent Technologies (1 mentions) Vimentin, by Roche (1 mentions)

Close spelling variants of this product

S100 protein (clone 4C4.9; (2 citations)

5 protocols using s100 protein

1

Immunohistochemical Profiling of NTRK-Rearranged Thyroid Tumors

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IHC stains were performed using 4-μm-thick formalin-fixed paraffin-embedded (FFPE) sections of selected representative tissue blocks from the 11 NTRK-rearranged thyroid tumors. A Bond 3 automated stainer (Leica Microsystems, Bannockburn, IL) and compatible primary antibodies for S100 protein (polyclonal and prediluted; Ventana, Oro Valley, AZ), mammaglobin (clone 304-1A5; dilution 1:250; Dako, Carpinteria, CA), GATA3 (clone L50-823, dilution 1:200; Cell Marque, Rocklin, CA), PAX8(polyclonal, dilution 1:1000; Proteintech, Rosemont, IL), TTF1 (clone 8G7G3/1; dilution 1:300; Dako, Carpinteria, CA), BRAF V600E (clone VE1; dilution 1:200; Abcam, Cambridge, United Kingdom), thyroglobulin (dilution 1:800; Cell Marque, Rocklin, CA) and HBME1 (prediluted; Ventana, Oro Valley, AZ) were used. Appropriate positive and negative controls were included. Stained slides were reviewed by three pathologists (VN, PMS, and YHC) and graded as negative (0% tumor cells stained), focal (<50%) and diffuse (>50%).
Chu Y.H., Dias-Santagata D., Farahani A.A., Boyraz B., Faquin W.C., Nosé V, & Sadow P.M. (2020). Clinicopathologic and molecular characterization of NTRK-rearranged thyroid carcinoma (NRTC). Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(11), 2186-2197.
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2

Immunohistochemical Profiling of Tumor Markers

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Immunohistochemical labeling was performed on the Benchmark XT autostainer (Ventana Medical Systems Inc, Tucson, AZ) using the I-View detection kit. The standard antibodies used, vendors, pretreatments, and dilutions included: cytokeratin AE1/3 (Chemicon, steam, 1:4000), EMA (Ventana, 760–4259, steam, prediluted), WT1 (Cell Marque, clone 6F-H2, Steam, Prediluted), Calretinin (Biocare, Steam, Prediluted polyclonal), desmin (Dako M0760, clone D33, steam, 1:100), CD99 (Leica, Clone 12E7, steam, prediluted), S100 protein (Ventana, 760–2914, stream, prediluted), BAP-1 (Santa Cruz, clone C-4, steam, 1:200), and INI-1 (BD Transduction Laboratories, clone 25/BAF47, steam, 1:100).
Argani P., Harvey I., Nielsen G.P., Takano A., Suurmeijer A.J., Voltaggio L., Zhang L., Sung Y.S., Stenzinger A., Mechtersheimer G., Dickson B.C, & Antonescu C.R. (2020). EWSR1/FUS-CREB Fusions Define a Distinctive Malignant Epithelioid Neoplasm with Predilection for Mesothelial-Lined Cavities. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(11), 2233-2243.
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3

Immunohistochemical Profiling of Tumor Markers

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Immunohistochemical labeling was performed on the Benchmark XT autostainer (Ventana Medical Systems Inc, Tucson, AZ) using the I-View detection kit. The standard antibodies used, vendors, pretreatments, and dilutions included: cytokeratin AE1/3 (Chemicon, steam, 1:4000), EMA (Ventana, 760–4259, steam, prediluted), WT1 (Cell Marque, clone 6F-H2, Steam, Prediluted), Calretinin (Biocare, Steam, Prediluted polyclonal), desmin (Dako M0760, clone D33, steam, 1:100), CD99 (Leica, Clone 12E7, steam, prediluted), S100 protein (Ventana, 760–2914, stream, prediluted), BAP-1 (Santa Cruz, clone C-4, steam, 1:200), and INI-1 (BD Transduction Laboratories, clone 25/BAF47, steam, 1:100).
Argani P., Harvey I., Nielsen G.P., Takano A., Suurmeijer A.J., Voltaggio L., Zhang L., Sung Y.S., Stenzinger A., Mechtersheimer G., Dickson B.C, & Antonescu C.R. (2020). EWSR1/FUS-CREB Fusions Define a Distinctive Malignant Epithelioid Neoplasm with Predilection for Mesothelial-Lined Cavities. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(11), 2233-2243.
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4

Immunohistochemical Profiling of Tumor Samples

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At Johns Hopkins University, immunohistochemical labeling was performed on the Benchmark XT autostainer (Ventana Medical Systems Inc, Tucson, AZ) using the I-View detection kit. The standard antibodies used, vendors, pretreatments, and dilutions were as follows: desmin (Dako M0760, clone D33, steam, 1:100), cytokeratin AE1/3 (Chemicon, steam, 1:4000) vimentin (Ventana, 790-2917, prediluted), EMA (Ventana, 760-4259, steam, prediluted), CD99 (Leica, Clone 12E7, steam, prediluted), Bcl2 (Leica, ORG-8714, steam, prediluted), S100 protein (Ventana, 760-2914, stream, prediluted), and CD34 (Immunotech/Coulter, catalog 0786, steam, prediluted). MUC4 immunohistochemical staining was performed at Memorial Sloan Kettering Cancer Center using anti-human mouse monoclonal antibody clone 8G7 at a dilution of 0.1 ug/ml (Santa Cruz Biotechnology, Santa Cruz, CA).
Argani P., Lewin J.R., Edmonds P., Netto G.J., Prieto-Granada C., Zhang L., Jungbluth A.A, & Antonescu C.R. (2015). Primary Renal Sclerosing Epithelioid Fibrosarcoma: Report of Two Cases with EWSR1-CREB3L1 Gene Fusion. The American journal of surgical pathology, 39(3), 365-373.
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5

Comprehensive Immunohistochemical Profiling

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Immunohistochemical labeling was performed on the Benchmark XT autostainer (Ventana Medical Systems Inc, Tucson, AZ) using the I-View detection kit. The standard antibodies used, vendors, pretreatments, and dilutions included: ALK (Abcam ab17127, 5A4, steam, 1:50), PAX8 (Proteintech 10336–1-AP, polyclonal, steam, 1:800), CK5/6 (Ventana/Roche 790–4554, D5/16B4, steam, predilute), BRG1 (Abcam ab110641, EPNCIR111A, steam, 1:100), desmin (Dako M0760, clone D33, steam, 1:100), cytokeratin AE1/3 (Chemicon, steam, 1:4000), EMA (Ventana, 760–4259, steam, prediluted), CD99 (Leica, Clone 12E7, steam, prediluted), S100 protein (Ventana, 760–2914, stream, prediluted), WT-1 (Ventana, 6F-H2, steam, predilute), calretinin (Cell Marque, polyclonal, steam, predilute), BAP1 (Santa Cruz, clone C4, steam, 1:200), and INI1 (BD Transduction Laboratories, clone 25/BAP47, steam, 1:400).
Argani P., Lian D.W., Agaimy A., Metzler M., Wobker S.E., Matoso A., Epstein J.I., Sung Y.S., Zhang L, & Antonescu C.R. (2021). Pediatric Mesothelioma with ALK Fusions: A Molecular and Pathologic Study of 5 Cases. The American journal of surgical pathology, 45(5), 653-661.
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