Standard flow cytometry was used as described in [16 (link)] for testing IFN-γ expression and CD8+/CD4+ T cell memory response. The following conjugated antibodies from eBiosciences (San Diego, CA, USA) were used: CD3-APC (145-2C11), CD4-PE (GK1.5), CD8-PerCP (53-6.7), CD44-AF780 (IM7), CD127-FITC, and CD44hiCD127+. After washing, splenocytes were fixed before being measured on a BD LSR II flow cytometer (BD Biosciences) and analyzed using FlowJo software 10.0 (Tree Star, Ashland, OR, USA). The MHC class II tetramers, which present AS15 to T cells, were obtained from the NIH tetramer facility and used as described previously.
Cd8 percp 53 6.7
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
The CD8-PerCP (53-6.7) is a fluorescently-labeled antibody used for the detection and analysis of CD8-positive cells in flow cytometry applications. The PerCP (Peridinin Chlorophyll Protein) fluorescent dye is conjugated to the CD8 antibody, allowing for the identification and quantification of CD8-expressing cells within a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cd8 percp 53 6.7
1
Flow Cytometry Analysis of T Cell Responses
2
Activation of Naive T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Naïve cells from LNs or Ag-experienced cell cultures were washed once and resuspended in IMDM medium supplemented with 5% FCS, l -glutamine, 100 U/mL penicillin and streptomycin and 50 μM β-mercaptoethanol. Triplicate cultures at 5 × 105 cells per well were added in 50 μL to 96-well plates containing media supplemented with NP68 and with or without CD8α-specific Fab fragments and incubated for 3 h at 37°/5%CO2. Stimulated cells were stained with CD8-PerCP (53–6.7), TCRβ-FITC (H57-597) and CD69-FITC (H1.2F3) specific mAbs (eBioscience, Hatfield, UK). A minimum of 30,000 events was collected using an LSRII (BD Biosciences, Oxford, UK), and data were analysed with FlowJo software (Tree Star, Olten, Switzerland).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!