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3 protocols using ethylenediamine tetraacetic acid disodium

1

Synthesis of Nanomaterials from Inorganic Salts

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FeCl2·4H2O, Fe2(SO4)3∙H2O, 25% ammonia solution, ethanol, octylamine, 1-dodecanethiol, trioctylphosphine, methylene blue, methyl orange, ethylenediamine tetraacetic acid disodium, ascorbic acid, silver nitrate, isopropanol, hydrochloric acid, and sodium hydroxide were purchased from Merck (Darmstadt, Germany). All the reagents were used as purchased without further purification.
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2

Polyacrylamide Gel Electrophoresis Protocol

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Acetic acid ca. 100% (Bang & Co.) or Emprove (Merck). Acrylamide (99.9%) and N,N′-methylene-bis-Acrylamide (electrophoresis purity) (Bio-Rad). Agarose (GTG Agarose, Biometra). Ammonium persulfate (electrophoresis purity) (Bio-Rad). β-Mercaptoethanol puriss (Fluka). Boric acid p.a. (Merck). Bromophenol blue (Merck). Coomassie Brilliant Blue R 250 (Serva). Cytochrome-C, type III (Sigma C-2506). DL-dithiothreitol (Sigma D-0632). DNA from Calf Thymus Type I, Na salt, highly polymerized, (Sigma D-1501). Ethidium bromide (Sigma E8751). Ethylenediamine tetraacetic acid disodium salt p.a. (Merck). Gel Drying Films (Promega). Glycine p.a. (Riedel-deHaën 33226). Methanol p.a. or SeccoSolv (Merck). Myoglobin from Horse Heart (Sigma M-9267). Plasmid pCaMVCN (Pharmacia) 4177 bp, linearized with Cla I (NEB). Precision Protein Standards (Bio-Rad 161-0362) with given molar masses of 250, 150, 100, 75, 50, 37, 25, 15, & 10 kDa. 2-propanol, p.a. Emsure or SeccoSolv (Merck). RNA, soluble from Yeast, Type III (Sigma R-7125). Sodium dodecyl sulphate (BDH 44215). TEMED (electrophoresis purity) (Bio-Rad). Tris, Trizma preset pH, base and HCl (Sigma).
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3

Neurochemical Analysis of MPTP Model

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Dopamine, sodium 1-octanesulfonate monohydrate, 5-HT, HVA, 5-HIAA, sodium metabisulfite and DOPAC were obtained from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Sodium chloride, hydrochloric acid, sodium dihydrogen phosphate, ethylenediaminetetraacetic acid disodium, sodium hydroxide, and acetonitrile were purchased from E. Merck (Darmstadt, Germany). MPTP hydrochloride was purchased from T.C.I. Co., LTD. (Tokyo, Japan)
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