The largest database of trusted experimental protocols

Anti cd20

Manufactured by Cell Signaling Technology
Sourced in United States

Anti-CD20 is a laboratory reagent used for the detection and analysis of the CD20 protein, which is expressed on the surface of B cells. This product is intended for research use only and its specific function is to bind to the CD20 protein, allowing for the identification and quantification of B cells in various experimental and analytical applications.

Automatically generated - may contain errors

2 protocols using anti cd20

1

Double Immunofluorescence Staining of B-Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Double immunofluorescence staining was performed as previously described.36 (link) Briefly, B-cells were fixed in 4% paraformaldehyde (15 min, RT), washed with PBS, permeabilized (0.1% Triton X-100 in PBS, 15 min), and attached on pre-coated poly-L-lysine slides. Slides were blocked in 3% BSA, followed by mouse monoclonal anti-NS3 (Abcam; 1:100, ab65407) or anti-core (ThermoFisher Scientific, Waltham, MA, USA; 1:100, MA1-080) antibody staining. The cells were further incubated with rabbit polyclonal anti-CD19, anti-CD20 (Cell Signaling Technology, Inc., Danvers, MA, USA; 1:400; 3574), anti-CD20 (Abcam; 1:400; ab78237) or anti-B220 antibody (Biolegend, San Diego, CA, USA; 1:200; #103201). Cells were washed with PBS and incubated with goat anti-rabbit Alexa Fluor 488 and goat anti-mouse or anti-rat Alexa Fluor 597 (Invitrogen; 1:200). The stained slides were mounted using Vectashield mounting medium (Vector Laboratories Inc., Burlingame, CA, USA). Control slides were similarly processed, except primary antisera were omitted, which yielded no staining. Images were captured using an Olympus FV1000 confocal microscope and processed with Olympus Fluoview Version 1.7c software (Olympus Corporation, Tokyo, Japan).
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Lymph Nodes

Check if the same lab product or an alternative is used in the 5 most similar protocols
H&E staining and IHC were performed on formalin-fixed and paraffin-embedded LNs, which included nine enlarged and five non-enlarged LNs. All LNs were dissected transversely in the middle of the longitudinal axis and serially sectioned. The primary antibodies used were anti-CD20 (Cell Signaling Technology, 48750T, 1: 100) for identifying B cells, anti-CD68 (Cell Signaling Technology, 76437T) for identifying macrophages, anti-CD3 (Servicebio, GB11014) for identifying T cells, and anti-Ki67(Cell Signaling Technology, 9449T) for identifying proliferating cells (18 (link)). Diluents without primary antibodies were used as negative controls. Staining was visualized using the Dako REAL™ EnVision™ Detection System followed by counterstaining with hematoxylin. Images were captured using a digital camera under a light microscope (VS120; Olympus). The number of germinal centers (GCs) observed was counted in one HE-stained section. The proportions of CD68- and Ki67-positive cells in the LNs were calculated as positive cells versus total cells in at least five randomly selected areas under ×1000 magnification of microscopic fields.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!