Sep pak qma carbonate plus light cartridges

No-carrier-added
aqueous [¹⁸F]fluoride was produced via the ¹⁸O(p,n)¹⁸F nuclear reaction by irradiation of ¹⁸O-enriched water by a GE PETtrace 8 cyclotron. All radiofluorination
reactions were carried out on a GE TRACERlab FX_FN automated
synthesizer. Sep-Pak QMA Carbonate Plus Light cartridges (Waters)
were preconditioned with water (10 mL) prior to use. Oasis MCX Plus
Short (Waters) and Bond Elut SCX 1 g (Agilent) cartridges were preconditioned
with ethanol (5 mL) and then with water (10 mL) prior to use. The
starting activity for calculating the radiochemical yield was determined
from the GM reading taken immediately following delivery of [¹⁸F]fluoride to the synthesizer from the cyclotron. The final
activity readings were recorded using a Capintec CRC-25 PET dose calibrator.

No-carrier-added
aqueous [¹⁸F]fluoride was produced via the ¹⁸O(p,n)¹⁸F nuclear reaction by irradiation of ¹⁸O-enriched water by a GE PETtrace 8 cyclotron. All radiofluorination
reactions were carried out on a GE TRACERlab FXFN automated synthesizer.
Sep-Pak QMA Carbonate Plus Light cartridges (Waters) were preconditioned
with water (10 mL) prior to use. Oasis HLB Plus Light (Waters) cartridges
were preconditioned with ethanol (5 mL) and then with water (10 mL)
prior to use. The starting activity for calculating the RCY was determined
from the Geiger–Müller (GM) reading taken immediately
following the delivery of [¹⁸F]fluoride to the synthesizer
from the cyclotron. The final activity readings were recorded using
a Capintec CRC-25 PET dose calibrator. Analytical HPLC was carried
out on a Thermo Dionex Ulimate system 3000 equipped with a Berthold
FlowStar LB 513 radio flow detector and a DAD-3000 UV detector. An
isocratic mobile phase of 60% acetonitrile in water was used with
an Agilent Pursuit XRs 5 C18 250 mm × 4.0 mm column at a rate
of 1 mL min^–1. The nonradioactive standards were
detected using a UV wavelength of 267 nm.