The splenocytes were treated with fluorescein (FITC) anti-CD11c antibody (clone: N418), PE-cy7 anti-CD11b antibody (clone: M1/70), PE-cy5 anti-CD3e antibody (clone: 145-2C11), FITC anti-CD8a antibody (clone: 5H10-1), antigen-presenting cell (APC) anti-F4/80 antibody (clone: BM8), FITC anti-CD206 antibody (clone: C068C2), and APC anti-CD4 antibody (clone: GK1.5) (BioLegend, Seoul, Korea). The expression of CD3e, CD11b, CD11c, CD8a, and CD4 in splenocytes was analyzed using flow cytometry (FACSCalibur, BD Bioscience, Korea).
Fitc anti cd8a antibody
Manufactured by BioLegend
The FITC anti-CD8a antibody is a flow cytometry reagent used for the identification and enumeration of CD8a-positive cells. It binds specifically to the CD8a cell surface antigen, which is expressed on a subset of T lymphocytes. The FITC fluorophore is conjugated to the antibody, allowing for detection and analysis of CD8a-positive cells by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Anti f4 80 apc antibody, by BioLegend (1 mentions)
Fitc anti cd11c antibody, by BioLegend (1 mentions)
Fitc anti cd206 antibody, by BioLegend (1 mentions)
Facscalibur, by BD (1 mentions)
Recombinant mouse granulocyte macrophage colony stimulating factor gm csf, by Thermo Fisher Scientific (1 mentions)
Interleukin il 4, by Thermo Fisher Scientific (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Fitc anti cd4 antibody, by BioLegend (1 mentions)
Anti ifn γ apc antibody, by BioLegend (1 mentions)
Rpmi medium 1640 basic, by Thermo Fisher Scientific (1 mentions)
Brefeldin a solution, by BioLegend (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Mouse cytokine elisa kits, by BioLegend (1 mentions)
2 protocols using fitc anti cd8a antibody
1
Splenocyte Immunophenotyping by Flow Cytometry
2
Activation of Mouse T-Cell Subsets
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Ovalbumin was obtained from Sigma-Aldrich (St. Louis, MO, USA). Brefeldin A solution and the following fluorescence-labeled antibodies, mouse PE/Cy7-anti-CD3 antibody, FITC-anti-CD4 antibody, FITC-anti-CD8a antibody, Percp-anti-CD8a antibody, PE-anti-IL4 antibody and APC-anti-IFN-γ antibody, were purchased from BioLegend (San Diego, CA). LysoTracker Red DND-99, RPMI medium 1640 basic, fetal bovine serum and penicillin streptomycin were purchased from Thermo Fisher Scientific (MA, USA). Mouse cytokine ELISA kits were obtained from BioLegend (San Diego, CA, USA). Recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 were purchased from PeproTech (Rocky Hill, USA).
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