Uptake assays were performed as previously described [15 ]. Briefly, U87 cells were plated 1 day prior at 50 % confluency in 15-cm tissue culture-treated plates. On the day of the experiment, media was replaced with 15 ml of media conditioned to 37 °C and 5 % CO2, supplemented with 185 kBq/ml F-18 labeled probe, 0.37 kBq/ml [14C]FMAU, or 37 kBq/ml [124I]FIAU and placed in a cell incubator at 37 °C and 5 % CO2 for 2 h. Cells were collected by scraping and centrifugation, and washed twice with ice-cold PBS. For F-18 and I-124 labeled assays, cell pellets and corresponding supernatant samples were counted on a Wallac Wizard 3″ 1480 Automatic Gamma Counter (PerkinElmer). For [14C]FMAU, cell pellets and supernatant samples were resuspended in Insta-Fluor scintillation liquid (Perkin Elmer), vortexed and counted on a TriCarb 2910 Scintillation Counter (PerkinElmer). Cell pellets were normalized to their respective masses.
Wallac wizard 3 1480 automatic gamma counter
Manufactured by PerkinElmer
Sourced in United States
The Wallac Wizard 3'' 1480 Automatic Gamma Counter is a laboratory equipment designed for the measurement and analysis of gamma radiation. It features automatic sample handling and data processing capabilities.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using wallac wizard 3 1480 automatic gamma counter
1
Uptake Assays for Radiolabeled Probes
2
Cytotoxicity Assay for Effector T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cytotoxicity of effector T cells was measured by a standard 4-h 51Cr-release assay that used T24 cells, K562 cells, or tumor antigen-pulsed DCs as targets. Effector cells were incubated with 51Cr-labeled target cells (5 × 103) for 4 h at 37 °C in 200 µL of RPMI 1640 medium containing 10% FCS in round-bottomed 96-well cell culture plates. After incubation, the plates were centrifuged for 10 min at 330×g, and 100 µL of cell-free supernatant was collected to measure radioactivity with a Wallac Wizard 3 (1480 Automatic Gamma Counter; Perkin Elmer, Waltham, MA, USA). Maximum release was determined from the supernatant of cells that had been lysed by the addition of 5% Triton X-100, and spontaneous release was determined from target cells incubated without added effector cells. The percent specific lysis was calculated as follows: 100 × (experimental release − spontaneous release)/(maximum release − spontaneous release).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!