Anti 14 3 3θ

Manufactured by Santa Cruz Biotechnology

Anti-14-3-3θ is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that specifically detects the 14-3-3θ protein.

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Close spelling variants of this product

Anti-14-3-3θ antibody Anti-14-3-3

2 protocols using anti 14 3 3θ

Immunoprecipitation and Immunoblotting Assay

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Cell lysates were prepared in modified RIPA buffer and incubated overnight at 4°C, with specific antibodies. The immunocomplexes were isolated using protein-A Sepharose (Incospharm), resolved by SDS-PAGE, and transferred to nitrocellulose membrane. Immunoblotting was performed with various antibodies. Anti-ICP27, anti-Bax, anti-14-3-3θ, anti-cytochrome c, anti-COX IV, anti-α-tubulin, anti-β-actin, anti-acetylated lysine, anti-SIRT2, goat anti-rabbit IgG-HRP, rabbit anti-goat IgG-HRP, and goat anti-mouse IgG-HRP antibodies were purchased from Santa Cruz. Anti-Flag and anti-GST antibodies were purchased from Sigma-Aldrich.

Kim J.A., Kim J.C., Min J.S., Kang I., Oh J, & Ahn J.K. (2017). HSV-1 ICP27 induces apoptosis by promoting Bax translocation to mitochondria through interacting with 14-3-3θ. BMB Reports, 50(5), 257-262.

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Antibody Panel for Protein Analysis

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Anti‐14‐3‐3θ, sc‐632; anti‐α‐tubulin, sc‐5286; anti‐vinculin, sc‐25336 (Santa Cruz Biotechnology); anti‐AF4, #A302‐344A (Bethyl Laboratories); anti‐MLL^N, #05‐764; anti‐MLL^C, #05‐765 (Merck Millipore); anti‐RUNX1, #4336 (Cell Signaling Technology); horseradish peroxidase‐conjugated anti‐mouse (#NA931) and anti‐rabbit (#NA934) IgG secondary antibodies (GE Healthcare).

Fioretti T., Zanobio M., Raia M., Errichiello S., Izzo B., Cattaneo F., Ammendola R., Cevenini A, & Esposito G. (2022). MiR‐27a downregulates 14‐3‐3θ, RUNX1, AF4, and MLL‐AF4, crucial drivers of blast transformation in t(4;11) leukemia cells. Cell Biochemistry and Function, 40(7), 706-717.

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